Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

P7965

Sigma-Aldrich

Monoclonal Anti-P-Glycoprotein (MDR) antibody produced in mouse

clone F4, ascites fluid

Synonyme(s) :

Anti-ABC20, Anti-CD243, Anti-CLCS, Anti-GP170, Anti-MDR1, Anti-P-GP, Anti-PGY1, Anti-p-170

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

ascites fluid

Type de produit anticorps

primary antibodies

Clone

F4, monoclonal

Poids mol.

antigen 170-180 kDa

Contient

15 mM sodium azide

Espèces réactives

hamster, human

Technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human kidney sections
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
radioimmunoassay: suitable using cell-surface RIA
western blot: suitable

Isotype

IgG1

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... ABCB1(5243)

Description générale

The ABCB1 (ATP binding cassette subfamily B member 1) gene is mapped to human chromosome 7q21.12. It encodes for P-glycoprotein, which is a membrane bound drug transporter protein, belonging to the ATP-binding cassette (ABC) transporters family. The gene is considered to be highly polymorphic.

Spécificité

The antibody recognizes an epitope located in the amino terminal half of P-glycoprotein (Pgp), at the third extracellular loop of the molecule. The epitope is resistant to formalin fixation and periodate oxidation. The antibody detects specifically human MDR1 P-glycoprotein, but does not appear to recognize the human MDR3 product, nor the mouse mdr1a, mdr1b or the mdr3 P-glycoprotein.

Immunogène

mixture of human and hamster drug-resistant whole cells and crude plasma membranes.

Application

Monoclonal Anti-P-Glycoprotein (MDR) antibody produced in mouse has been used in western blot analysis and Immunohistochemistry.

Actions biochimiques/physiologiques

Overexpression of ABCB1 (ATP binding cassette subfamily B member 1) gene is a major cause for multi-drug resistance, which makes chemotherapy challenging for the treatment of osteosarcoma. P-glycoprotein (P-gp) mediates the energy-dependent efflux of xenobiotic to the outside of plasma membrane from the inside of the cell , thereby affecting the process of absorption, distribution, and excretion of drugs. Cyclosporine, a calcineurin inhibitor serves as a substrate for P-gp.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Zhi Shi et al.
Biochemical pharmacology, 77(5), 781-793 (2008-12-09)
The tyrphostin 4-(3-chloroanilino)-6,7-dimethoxyquinazoline (AG1478) is a potent and specific EGFR tyrosine kinase inhibitor (TKI); its promising pre-clinical results have led to clinical trials. Overexpression of ATP-binding cassette (ABC) transporters such as ABCB1, ABCC1 and ABCG2 is one of the main
Di-2-pyridylketone 4, 4-Dimethyl-3-thiosemicarbazone (Dp44mT) Overcomes Multidrug-Resistance by a Novel Mechanism Involving the Hijacking of Lysosomal P-Glycoprotein (Pgp).
Jansson P J, et al.
The Journal of Biological Chemistry (2015)
P-glycoprotein mediates drug resistance via a novel mechanism involving lysosomal sequestration.
Yamagishi T, et al.
The Journal of Biological Chemistry (2013)
Effect of MDR1 C1236T polymorphism on cyclosporine pharmacokinetics: A systematic review and meta-analysis.
Chen Z, et al.
Medicine, 96(47) (2017)
Histone deacetylase inhibitors regulate P-gp expression in colorectal cancer via transcriptional activation and mRNA stabilization.
Wang H, et al.
Oncotarget, 7(31), 49848-49848 (2016)

Articles

We presents an article on ABC Transporters and Cancer Drug Resistance

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique