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N7653

Sigma-Aldrich

p-Nitrophenyl Phosphate Liquid Substrate System

alkaline phosphatase substrate, liquid

Synonyme(s) :

4-Nitrophenyl phosphate disodium salt solution, pNPP

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About This Item

Formule linéaire :
O2NC6H4OP(O)(ONa)2
Numéro CAS:
4264-83-9
Poids moléculaire :
263.05
Numéro MDL:
MFCD00007319
Code UNSPSC :
12352204
ID de substance PubChem :
24897835
Nomenclature NACRES :
NA.83

product name

p-Nitrophenyl Phosphate Liquid Substrate System, liquid

Forme

liquid

Niveau de qualité

200

Conditions d'expédition

wet ice

Température de stockage

−20°C

Chaîne SMILES 

[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2

Clé InChI

VIYFPAMJCJLZKD-UHFFFAOYSA-L

Description générale

The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.

Application

p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase in alkaline phosphatase assay.
Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Actions biochimiques/physiologiques

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP produces p-nitrophenol. It is a common chromogenic substrate for alkaline phosphatase.

Attention

Stable at least one year when stored below 0 °C.

Forme physique

Ready-to-use.

Pictogrammes

Health hazardCorrosion
GHS08,GHS05

Mention d'avertissement

Danger

Mentions de danger

H290,H315,H318,H361fd,H373

Classification des risques

Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

Organes cibles

Kidney,Liver,Blood

Code de la classe de stockage

8B - Non-combustible corrosive hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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L Jonasson et al.
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European journal of medicinal chemistry, 178, 108-115 (2019-06-09)
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Proceedings of the National Academy of Sciences of the United States of America, 105(33), 11661-11666 (2008-08-12)
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International journal of biomedical research, 80(8), 467-474 (2007)
Afficher tous les articles scientifiques apparentés

Articles

High-throughput Screening of Peptide Substrates for Tyrosine Kinases Using PEPscreen® Custom Peptide Library

Review a high-throughput ELISA-based method to identify peptide substrates for class-specific and/or enzyme-specific Protein Tyrosine Kinases (PTKs) that can be utilized for the detection of kinase activity both in vivo and in vitro.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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