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Key Documents

N1142

Sigma-Aldrich

Neomycin

solution, suitable for cell culture, BioReagent

Synonyme(s) :

Antibiotic 10676, Neomycin B

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About This Item

Code UNSPSC :
12352207
ID de substance PubChem :
Nomenclature NACRES :
NA.76

product name

Neomycin solution, with 10 mg/mL neomycin in 0.9% NaCl, 0.1 μm filtered, BioReagent, suitable for cell culture

Source biologique

Streptomyces fradiae

Niveau de qualité

Stérilité

0.1 μm filtered

Gamme de produits

BioReagent

Forme

liquid

Puissance

8-12 mg per mL

Conditionnement

pkg of 20ML

Concentration

10 mg/mL in 0.9% NaCl (neomycin)

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Couleur

clear

Numéro d'accès UniProt

Spectre d'activité de l'antibiotique

Gram-negative bacteria
Gram-positive bacteria

Mode d’action

protein synthesis | interferes

Température de stockage

2-8°C

Chaîne SMILES 

NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O)[C@H]2O[C@@H]3O[C@H](CO)[C@@H](O[C@H]4O[C@@H](CN)[C@@H](O)[C@H](O)[C@H]4N)[C@H]3O)[C@H](N)[C@@H](O)[C@@H]1O

Informations sur le gène

human ... TERT(7015)

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Description générale

Chemical structure: aminoglycoside

Application

Neomycin solution has been used:
  • as a supplement of autoclaved acidified water for bone marrow transplantation
  • for the selection of stable clones
  • as a component of artificial sea water (ASWO) medium to remove bacteria from O. tauri cultures

Actions biochimiques/physiologiques

Neomycin is an aminoglycoside antibiotic, produced by Streptomyces containing a minimum of 85% neomycin B. It acts as a selection agent for prokaryotic cells transformed using the neo selectable marker gene. Neomycin trisulfate is used to study the cytototoxic side effects of antibiotics, platelet-derived growth factor responses in certain fibroblasts and extraction of nuclear phosphatidylinositol 4,5-bisphosphate-interacting proteins. This product is recommended for use in cell culture applications at 5 mL/L.
Mode of action: This product acts by binding to the 30S and 50S subunits, causing miscoding and inhibiting initiation and elongation during protein synthesis. Neomycin also blocks voltage-sensitive Ca2+ channels without affecting the Na+/Ca2+ antiporter in neurons.
Antimicrobial spectrum: Neomycin acts against both gram-positive and gram-negative bacteria

Attention

This solution is formulated to contain 10 mg/mL neomycin in 0.9% sodium chloride. It is stable at 37 °C for 5 days and should be otherwise stored at 2-8°C.

Pictogrammes

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Tudor Arvinte et al.
Journal of pharmaceutical and biomedical analysis, 176, 112802-112802 (2019-08-26)
The physicochemical properties of Avastin® manufactured in the USA (Originator USA) and in Europe (Originator EU) and ABX-BEV, a bevacizumab biosimilar drug product candidate produced by Apobiologix Inc., were characterized at a clinically relevant concentration of 2 mg/mL following dilution of
Long non-coding antisense RNA controls Uchl1 translation through an embedded SINEB2 repeat
Carrieri C, et al.
Nature, 491(7424), 454-454 (2012)
Metabolic profiling identifies trehalose as an abundant and diurnally fluctuating metabolite in the microalga Ostreococcus tauri
Hirth M, et al.
Metabolomics, 13(6), 68-68 (2017)
Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases (2014)
Bone marrow p16INK4a-deficiency does not modulate obesity, glucose homeostasis or atherosclerosis development
Wouters K, et al.
Testing, 7(3), e32440-e32440 (2012)

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Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic

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