MAK489

Glucose Uptake Assay Kit

sufficient for 100 fluorometric tests

• Code UNSPSC : 12161503

Propriétés

• Niveau de qualité: 100
• Application(s): pharmaceutical
• Méthode de détection: fluorometric
• Maladie(s) pertinente(s): endocrinological disorders, diabetes; cancer
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

Glucose uptake has a variety of methods and transporters and depends upon the metabolic demand of the cell type and availability of glucose. There are over ten different facilitated diffusion glucose transporters which transports glucose down its concentration gradient without ATP hydrolysis. In the kidneys, secondary active transport is used to uptake glucose against its concentration gradient to ensure that very little glucose is excreted in urine.

Caractéristiques et avantages

• Linear detection range: 0.1 µM to 5 µM 2-DG6P

Adéquation

The kit is suitable for the determination of glucose uptake in whole cells and the evaluation of effects of ligands or drugs on glucose transport.

Principe

The Glucose Uptake Assay Kit is a fluorescent cell-based assay that uses 2-deoxyglucose (2-DG), a widely used glucose analog because it can be taken up by glucose transporters and metabolized by endogenous hexokinase, into 2-deoxyglucose 6-phosphate (2-DG6P). 2-DG6P accumulates intracellularly because it is not a suitable substrate for phosphoglucose isomerase, the next step in glycolysis. The cells are lysed, and excess NADP and glucose 6-phosphate dehydrogenase (G6PDH) is added to metabolize 2-DG6P and generate a molar equivalent amount of NADPH. The NADPH is then measured using a G6PDH recycling reaction to amplify the signal and generate a fluorescent signal measureable at λEx = 530 nm/λEm = 585 nm proportional to the concentration of 2-DG6P.

Informations sur la sécurité

• Pictogrammes: GHS05
• Mention d'avertissement: Danger
• Mentions de danger: H290 - H314
• Conseils de prudence: P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Classification des risques: Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B
• Code de la classe de stockage: 8A - Combustible, corrosive hazardous materials
• Classe de danger pour l'eau (WGK): WGK 3