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MAK447

Sigma-Aldrich

Alkaline Phosphatase Assay Kit

Sufficient for 250 colorimetric tests

Synonyme(s) :

ALP Activity Assay Kit

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About This Item

Code UNSPSC :
12161503

Niveau de qualité

100

Application(s)

pharmaceutical

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

osteoporosis; gastrointestinal diseases; orthopedic diseases; cancer

Température de stockage

−20°C

Catégories apparentées

Description générale

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis.

Application

The Alkalnine Phosphatase Assay Kit may be used for:
  • Cancer Research
  • Gastroenterology Disease Research
  • Osteoporosis Research

Caractéristiques et avantages

Enhanced Detection Range: Detect ALP levels accurately across a wide range, from 2 U/L to 800 U/L, using only 5 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.

Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a short 5 minute assay reaction, reducing complexity and saving valuable time and effort.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Adéquation

The kit is suitable for the quantitative determination of alkaline phosphatase (ALP) activity in serum, plasma etc.

Principe

The Alkaline Phosphatase Assay Kit is designed to measure ALP activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl phosphate that is hydrolyzed by ALP into a yellow colored product (maximal absorbance at A405 nm). The rate of the reaction isdirectly proportional to the enzyme activity.

Autres remarques

For additional information on our range of Biochemicals, please complete this form.

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Code de la classe de stockage

8A - Combustible, corrosive hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

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