MAK326

Indole Assay Kit

sufficient for 100 colorimetric tests

• Code UNSPSC : 12161503
• Nomenclature NACRES : NA.84

Propriétés

• Utilisation: sufficient for 100 colorimetric tests
• Application(s): cosmetics; food and beverages
• Méthode de détection: colorimetric
• Maladie(s) pertinente(s): gastrointestinal diseases
• Température de stockage: 2-8°C

Description

Description générale

Indole is the primary product of tryptophan breakdown by tryptophanase. Structurally, it is characterized by a benzene ring fused to a pyrrole ring, containing a five-membered nitrogen-containing ring. This simple structure forms the core of a vast array of natural and synthetic compounds with a wide range of biological activities. In bacteria, indole is a critical signaling molecule, produced as a metabolite during the breakdown of the amino acid tryptophan. It plays a pivotal role in bacterial behavior, influencing biofilm formation, spore germination, and the expression of virulence factors. In the context of metabolism in larger organisms, indole derivatives are integral to the functioning of the central nervous system, serving as precursors for the synthesis of neurotransmitters such as serotonin and melatonin. These compounds are consequential in regulating mood, appetite, sleep, and circadian rhythms. Indole metabolism in the gut microbiota has been implicated in maintaining the integrity of the intestinal epithelial barrier and modulating the immune response, reflecting the complexity and importance of this molecule in biological systems.

Indole is the primary product of tryptophan breakdown by tryptophanase. The indole test is commonly performed on bacteria to classify them on their ability to break down tryptophan to indole.

Application

The Nitric Oxide Assay Kit may be used for:

• Gastrointestinal Disease Research
• Bacterial Culture Analysis

Caractéristiques et avantages

Quick and Sensitive: Detect Indole levels ranging from 3 - 100 μM with a short 10 minute procedure, providing both convenience and sensitivity.

Simplified Process: Enjoy a hassle-free procedure that requires the addition of a single working reagent, saving you time and effort.

Adéquation

Suitable for indole determination in biological samples (e.g. indole produced by indole positive bacteria).

Principe

The indole assay kit is based on a modified version of Ehlrich′s and Kovac′s reagents, which reacts with indole to produce a colored compound at 565 nm. The intensity of this colored compound is directly proportional to the indole in the sample. Linear detection range from 3 to 100 μM indole in 96-well plate assay.

Autres remarques

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Informations sur la sécurité

• Pictogrammes: GHS02,GHS05,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H225,H290,H315,H317,H319,H335,H336
• Conseils de prudence: P210 - P233 - P234 - P280 - P303 + P361 + P353 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
• Organes cibles: Central nervous system, Respiratory system
• Code de la classe de stockage: 3 - Flammable liquids