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Merck
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Principaux documents

MAK277

Sigma-Aldrich

GAPDH Activity Assay Kit

sufficient for 100 colorimetric tests

Synonyme(s) :

Glyceraldehyde-3-phosphate Dehydrogenase Activity Assay Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

cancer

Température de stockage

−20°C

Description générale

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase; EC 1.2.1.12) catalyzes the conversion of Glyceraldehyde-3-Phosphate (GAP) to 1, 3-Bisphosphate Glycerate (BPG) and plays a key role in glycolysis. The enzyme is involved in cellular processes such as apoptosis, membrane trafficking, iron metabolism and nuclear translocation. GAPDH (housekeeping gene) expression is stable and constitutive. Deregulation of GAPDH activity is associated with abnormal cell proliferation and carcinogenesis. Accurate quantitation of GAPDH activity is important for diagnosing diseases and studying normal cellular physiology.

Application

GAPDH Activity Assay Kit has been used to measure the enzyme activity of glyceraldehyde-3-phosphate dehydrogenase in testis.

Caractéristiques et avantages

Compatible with high-throughput handling systems.

Adéquation

Suitable for use with various tissue and cell culture samples.

Principe

The GAPDH Activity Assay Kit provides a simple and sensitive method for monitoring GAPDH activity in various samples. GAPDH activity is determined in a coupled enzyme reaction in which GAP is converted to BPG by GAPDH. This results in a colorimetric (450 nm) product proportional to the enzymatic activity present. The assay is sensitive to 100 mUnits/mL. One unit of GAPDH activity is the amount of enzyme that will generate 1.0 mmole of NADH per minute at pH 7.2 at 37 °C.

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Danger

Mentions de danger

H314,H412

Classification des risques

Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1B

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

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Certificats d'analyse (COA)

Lot/Batch Number
7B23K06800
6D15K06800
SLBW0755

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Altered expression profile of glycolytic enzymes during testicular ischemia reperfusion injury is associated with the p53/TIGAR pathway: effect of fructose 1, 6-diphosphate.
May A L and Renno W M
PeerJ, 4, e2195-e2195 (2016)
Samir El Qaidi et al.
Scientific reports, 11(1), 3834-3834 (2021-02-17)
Type III secretion system effector proteins have primarily been characterized for their interactions with host cell proteins and their ability to disrupt host signaling pathways. We are testing the hypothesis that some effectors are active within the bacterium, where they
May Al-Maghrebi et al.
PeerJ, 4, e2195-e2195 (2016-07-22)
Background. Testicular ischemia reperfusion injury (tIRI) is considered the mechanism underlying the pathology of testicular torsion and detorsion. Left untreated, tIRI can induce testis dysfunction, damage to spermatogenesis and possible infertility. In this study, we aimed to assess the activities

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