MAK169

High Sensitivity Pyrophosphate Assay Kit

sufficient for 200 fluorometric tests

• Synonyme(s) : Pyrophosphate (PPi) Assay Kit
• Numéro CE : 200-664-3
• Code UNSPSC : 12161503
• Nomenclature NACRES : NA.84

Propriétés

• Utilisation: sufficient for 200 fluorometric tests
• Application(s): cosmetics; food and beverages
• Méthode de détection: fluorometric
• Maladie(s) pertinente(s): genitourinary disorders; rheumatological diseases
• Température de stockage: −20°C

Description

Description générale

Pyrophosphate (PP_i) is produced by a number of biochemical reactions such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation, and the formation of fatty acid-coenzyme A esters.

Adéquation

The Pyrophosphate Assay Kit provides a simple and direct flourescent microplate procedure for measuring pyrophosphate in a variety of samples.

Principe

The pyrophosphate concentration of a sample is determined by the use of a unique fluorogenic pyrophosphate sensor in which the presence of pyrophosphate results in the production of a fluorescent product (λ_ex =370/λ_em = 470 nm) proportional to the pyrophosphate present. This assay is simpler and more robust than traditional enzyme-based methods and is ideal for screening enzyme activity or enzyme inhibitors.

Informations sur la sécurité

• Pictogrammes: GHS05
• Mention d'avertissement: Danger
• Mentions de danger: H318
• Conseils de prudence: P280 - P305 + P351 + P338
• Classification des risques: Eye Dam. 1
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3