MAK151
Fluorometric Thiol Assay Kit
sufficient for 200 fluorometric tests
Synonyme(s) :
Thiol Fluorescent Assay Kit
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Produits recommandés
Utilisation
sufficient for 200 fluorometric tests
Méthode de détection
fluorometric
Maladie(s) pertinente(s)
endocrinological disorders, diabetes; cancer
Température de stockage
−20°C
Description générale
Thiol groups, found as free cysteine, glutathione (GSH), and cysteine residues in proteins, are involved in many biological processes. The disulfide bonds generated when the thiol groups of two cysteine residues are oxidized contribute to the tertiary or quatenary structure of a protein.
Application
Fluorometric Thiol Assay Kit has been used to determine the lipid peroxidation expressed as thiols in samples.
Adéquation
This kit is suitable for the detection of thiols in a variety of different biological samples including plasma, urine, and cell extracts. This kit is not optimized for measuring thiol groups in proteins.
Principe
The Fluorometric Thiol Quantitation Kit provides an ultrasensitive assay to quantitate the thiol content of small molecules. The proprietary thiol sensor generates a strongly fluorescent adduct (λex = 490/λem = 520 nm) upon reacting with a thiol-containing compound. In addition, both absorption and emission spectra of the thiol adduct are pH-independent, making this assay kit highly robust. The kit can detect as little as 1 picomole of cysteine or GSH in a 100 μL assay volume (10 nM). The assay can be performed conveniently in either a 96 or 384 multiwell plate format.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Scientific reports, 11(1), 19197-19197 (2021-09-30)
Recently it was proposed that the redox status of cysteines acts as a redox switch to regulate both the oligomeric status and the activity of human dUTPase. In a separate report, a human dUTPase point mutation, resulting in a tyrosine
Aging, 13(13), 17568-17591 (2021-07-07)
The homeostasis of the ocular lens is maintained by a microcirculation system propagated through gap junction channels. It is well established that the intercellular communications of the lens become deteriorative during aging. However, the molecular basis for this change in
Antioxidant Effects of a Hydroxytyrosol-Based Pharmaceutical Formulation on Body Composition, Metabolic State, and Gene Expression: A Randomized Double-Blinded, Placebo-Controlled Crossover Trial.
Oxidative Medicine and Cellular Longevity, 2017, 14-14 (2017)
The Science of the total environment, 757, 143648-143648 (2020-12-15)
The Windermere Humic Aqueous Model (WHAM) is often used for risk assessment of metals; WHAM can be used to estimate the potential bioavailability of dissolved metals, where metals complexed to dissolved organic matter (DOM) are expected to be less toxic
Aging, 12(13), 13594-13617 (2020-06-20)
The general transcription factor, CREB has been shown to play an essential role in promoting cell proliferation, neuronal survival and synaptic plasticity in the nervous system. However, its function in stress response remains to be elusive. In the present study
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique