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MAK070

Sigma-Aldrich

Oxaloacetate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 colorimetric or fluorometric tests

Méthode de détection

colorimetric
fluorometric

Maladie(s) pertinente(s)

cancer

Température de stockage

−20°C

Description générale

Oxaloacetate (OAA) is an intermediate of both the TCA cycle and gluconeogenesis. It precedes citrate which is formed by the citrate synthase-mediated condensation of acetyl-CoA and OAA. OAA is also formed by the deamidation of aspartate or condensation of CO2 with pyruvate or PEP. Since mammals do not possess the enzymatic machinery to form TCA cycle intermediates from acetyl-CoA, OAA is one of the anaplerotic entry points via pyruvate and pyruvate carboxykinase. The Oxaloacetate Assay Kit provides a simple, sensitive and rapid means of quantifying OAA in a variety of samples.

Application

Oxaloacetate Assay Kit has been used to determine the concentration of oxaloacetate in samples.

Adéquation

Suitable for animal tissues, cell culture (adherent or suspension cells), serum and saliva.

Principe

In this kit, oxaloacetate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (lex = 535/lem = 587 nm) product, proportional to the oxaloacetate present. Typical detection ranges for this kit are 2–10 nmole (colorimetric) and 0.2–1 nmole (fluorometric).

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Réf. du produit
Description
Tarif

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H317,H319,H334

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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The transcription factor Cabut coordinates energy metabolism and the circadian clock in response to sugar sensing.
Bartok O, Teesalu M, Ashwall-Fluss R, et al.
The Embo Journal, 34(11), 1538-1553 (2015)
Zheng Li et al.
Stem cells (Dayton, Ohio), 37(12), 1542-1555 (2019-10-02)
Mitochondrial phosphoenolpyruvate carboxykinase (PCK2) is a rate-limiting enzyme that plays critical roles in multiple physiological processes. The decompensation of PCK2 leads to various energy metabolic disorders. However, little is known regarding the effects of PCK2 on osteogenesis by human mesenchymal
Huabo Wang et al.
The Journal of biological chemistry, 294(14), 5466-5486 (2019-02-14)
Eukaryotic cell metabolism consists of processes that generate available energy, such as glycolysis, the tricarboxylic acid (TCA) cycle, and oxidative phosphorylation (Oxphos), and those that consume it, including macromolecular synthesis, the maintenance of ionic gradients, and cellular detoxification. By converting
Ke Tang et al.
Oncogene, 38(44), 6970-6984 (2019-08-15)
Clinical applications of antiangiogenic agents profoundly affect tumor cell behaviors via the resultant hypoxia. To date, how the hypoxia regulates tumor cells remains unclear. Here, we show that hypoxia promotes the growth of human breast tumorigenic cells that repopulate tumors
The transcription factor Cabut coordinates energy metabolism and the circadian clock in response to sugar sensing.
Bartok O, et al.
The Embo Journal, 34(11), 1538-1553 (2015)
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