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MAK069

Sigma-Aldrich

MPO Fluorometric Activity Assay Kit

sufficient for 100 fluorometric tests

Synonyme(s) :

Myeoloperoxidase Fluorometric Activity Assay Kit

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About This Item

Code UNSPSC :
12161503

Utilisation

sufficient for 100 fluorometric tests

Méthode de détection

fluorometric

Maladie(s) pertinente(s)

cancer; cardiovascular diseases; hematological disorder

Température de stockage

−20°C

Informations sur le gène

human ... MPO(4353)
mouse ... MPO(17523)
rat ... MPO(303413)

Description générale

Myeloperoxidase (MPO) is a heme-containing enzyme that catalyzes the hydrogen peroxidase-mediated oxidation of halide ions to hypohalous acid. MPO is a lysosomal protein, highly expressed in neutrophils, that plays a role in the antimicrobial actions that occur as a result of neutrophil stimulation. The actions of MPO may also contribute to the initiation and pathogenesis of cardiovascular disease.

Application

MPO Fluorometric Activity Assay Kit has been used to determine the activity of myeloperoxidase.

Adéquation

Suitable for the measurement of myeloperoxidase (MPO) in biological samples such as tissue, cells, serum and white blood cells

Principe

The MPO Activity Flurometric Assay kit provides a simple and direct procedure for measuring MPO activity in a variety of samples. In this assay, MPO catalyzes the formation of hypochlorous acid with reacts with the substrate, aminophenyl fluorescein, to generate fluorescein) (λex = 485/λem = 525 nm). One unit of MPO is the amount of enzyme that will oxidize the MPO substrate to yield 1.0 μmole of fluorescein per minute at room temperature.

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Classification des risques

Resp. Sens. 1

Code de la classe de stockage

10 - Combustible liquids


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