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Key Documents

M5524

Sigma-Aldrich

Murashige and Skoog Basal Salt Mixture (MS)

powder, suitable for plant cell culture

Synonyme(s) :

MS Basal Salts, MS0 Basal Salts, MSO Basal Salts

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.72

Forme

powder

Niveau de qualité

Technique(s)

cell culture | plant: suitable

Application(s)

agriculture

Conditions d'expédition

ambient

Température de stockage

2-8°C

Description générale

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and Kinetin (cytokinin/cell division promoter).

Application

Murashige and Skoog Basal Salt Mixture (MS) has been used to support the growth and regeneration of transformed tobacco cells. It has also been used as a growth medium for germination of Arabidopsis thaliana (Col-0) seeds.

Variante de formule

With the macro- and micronutrients as described by Murashige and Skoog (1962).

Media Formulation

Quantité

Formulated to contain 4.3 grams of powder per liter of medium.

Notes préparatoires

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Salt mixture (M5524) contains the macronutrients and micronutrients of the original classic formulation. It can be combined with M&S vitamins or Gamborg′s vitamins and supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

Autres remarques

Storage
Powdered media are extremely hygroscopic and must be protected from atmospheric moisture. If possible the entire contents of each package should be used immediately after opening.

Store dry medium in a desiccator at 0-5 °C.

Deterioration of powdered medium may be recognized by: 1) color change; 2) granulation, clumping, or particulate matter throughout the powder; 3) insolubility; 4) pH change; or 5) inability to promote growth when properly used.

Media Preparation

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

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Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Eye Irrit. 2 - Ox. Sol. 3

Code de la classe de stockage

5.1B - Oxidizing hazardous materials

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Pyae Phyo et al.
Plant physiology, 175(4), 1593-1607 (2017-11-01)
At early stages of Arabidopsis (
Joanna Banasiak et al.
Frontiers in plant science, 11, 18-18 (2020-03-03)
Strigolactones (SLs) are plant-derived signaling molecules that stimulate the hyphal branching of arbuscular mycorrhizal fungi (AMF), and consequently promote symbiotic interaction between the fungus and the plant. Currently, our knowledge on the molecular mechanism of SL transport is restricted to
Chien-Yu Huang et al.
Plant physiology, 174(4), 2248-2260 (2017-06-15)
Plant cytosolic lipid droplets (LDs) are covered with a layer of phospholipids and oleosin and were extensively studied before those in mammals and yeast. Oleosin has short amphipathic N- and C-terminal peptides flanking a conserved 72-residue hydrophobic hairpin, which penetrates
Songfeng Wang et al.
The Science of the total environment, 599-600, 332-339 (2017-05-10)
The nature and stability of bound residues (BRs) derived from the widely used brominated flame retardant tetrabromobisphenol A (TBBPA) in fine-textured soil is unknown. We incubated
Long Xiao et al.
BMC plant biology, 20(1), 292-292 (2020-06-27)
Starch is synthesized during daylight for temporary storage in leaves and then degraded during the subsequent night to support plant growth and development. Impairment of starch degradation leads to stunted growth, even senescence and death. The nuclear pore complex is

Articles

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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