M4401
Monoclonal Anti-Myosin (Light Chains 20 kDa) antibody produced in mouse
clone MY-21, ascites fluid
Synonyme(s) :
Myosin Light Chain Antibody, Myosin Light Chain Antibody - Monoclonal Anti-Myosin (Light Chains 20 kDa) antibody produced in mouse
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About This Item
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
ascites fluid
Type de produit anticorps
primary antibodies
Clone
MY-21, monoclonal
Poids mol.
antigen 20 kDa
Contient
15 mM sodium azide
Espèces réactives
human, chicken, pig, bovine, rabbit
Technique(s)
indirect immunofluorescence: 1:200 using human or chicken fibroblasts
western blot: suitable using denatured and reduced myosin of muscle
Isotype
IgM
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
chicken ... MYLK2(396356)
human ... MYLK2(85366)
Description générale
Monoclonal Anti-Myosin (Light Chains 20 kDa) (mouse IgM isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Myosin is a 500,000 Da protein known to interact with identical heavy chains (200,000 daltons each) and four light chains (15,000-20,000 daltons). Myosin molecules consist of two major regions: tail (rod) and head regions.
Spécificité
Monoclonal Anti-Myosin reacts best with the 20,000 dalton light chains of chicken gizzard, intestine or pig stomach myosin (SDS heat denatured and reduced).
Immunogène
chicken lens membrane.
Application
Monoclonal Anti-Myosin (Light Chains 20 kDa) antibody produced in mouse has been used in:
- western blot analysis
- fluorescence immunohistochemistry
- confocal microscopy
Monoclonal anti-myosin (Light Chains 20 kDa) antibody can be used in immunoblotting and western blotting.
Actions biochimiques/physiologiques
Myosin is a cytoskeletal protein responsible for muscle contraction, muscle filament sliding and regulation of ATPase activity. Monoclonal anti-myosin (Light Chains 20 kDa) antibody diluted 1:200 can be used to detect MLC20. It is also useful in staining (1:10,000) 2D slab gels. Anti-myosin(Light Chains 20 kDa) antibody reacts specifically with 20 kDa light chain of chicken gizzard, chicken intestine and pig stomach myosin which is SDS, heat denatured and reduced but not with intact myosin molecule in its native state.
The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Numerous investigators have shown that actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Myosin molecules spontaneously assemble into filaments in solutions of physiologic ionic strength and pH. Thick filaments consist mainly of myosin molecules. Myosin is an enzyme (ATPase) and its activity is the immediate source of the free energy that drives muscle contraction. Myosin binds to the polymerized form of actin, the major constituent of the thin filament.
Forme physique
The product is provided as ascites fluid with 15 mM sodium azide as a preservative.
Stockage et stabilité
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Toshihito Kakei et al.
Proceedings of the Japan Academy. Series B, Physical and biological sciences, 88(5), 201-211 (2012-06-13)
Chara myosin is plant myosin responsible for cytoplasmic streaming and moves actin filaments at 60 µm/s, which is the fastest of all myosins examined. The neck of the myosin molecule has usually mechanical and regulatory roles. The neck of Chara
Single Molecule Studies of Myosins
Farrow RE, et al.
Single Molecule Biology, 1-1 (2009)
Shaohua Chang et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 289(4), R1124-R1130 (2005-06-18)
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Electron microscopic studies of myosin molecules from chicken gizzard muscle I: the formation of the intramolecular loop in the myosin tail
Onishi H and Wakabayashi T
Journal of Biochemistry, 92(3), 871-879 (1982)
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