L5006

Leucine Aminopeptidase, microsomal from porcine kidney

Type IV-S, ammonium sulfate suspension, 10-40 units/mg protein (Bradford)

• Synonyme(s) : Aminopeptidase M, Aminopeptidase N, Cathepsin III, LAP, Leucinamide aminopeptidase, Leucinaminopeptidase
• Numéro CAS: 9054-63-1
• Numéro de classification (Commission des enzymes): 3.4.11.2 (BRENDA, IUBMB)
• Numéro CE : 232-942-5
• Numéro MDL: MFCD00131501
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Type: Type IV-S
• Niveau de qualité: 200
• Forme: ammonium sulfate suspension
• Activité spécifique: 10-40 units/mg protein (Bradford)
• Température de stockage: 2-8°C

Description

Description générale

Leucine Aminopeptidase belongs to the family of metalloprotease.

Application

Leucine Aminopeptidase, microsomal from porcine kidney has been used in the isolation of neprilysin (NEP) from rodent brain-C5 and for peptide digestion.

Actions biochimiques/physiologiques

Leucine Aminopeptidase possesses broad substrate-specificity. It hydrolyzes peptides up to the proline residue and does not continue to degrade beyond proline.

Définition de l'unité

One unit will hydrolyze 1.0 μmole of L-leucine-p-nitroanilide to L-leucine and p-nitroaniline per min at pH 7.2 at 37 °C. (At 25 °C, approx. 40% of the activity at 37 °C is obtained.) The activity obtained using L-leucine p-nitroanilide as substrate is 2-5 times that obtained with L-leucinamide as substrate.

Forme physique

Suspension in 3.5 M (NH₄)₂SO₄ solution, pH 7.7, containing 10 mM MgCl₂

Informations sur la sécurité

• Pictogrammes: GHS07,GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H315 - H319 - H334 - H335
• Conseils de prudence: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)