Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

View All Documentation

H8891

Sigma-Aldrich

Heparinase III from Flavobacterium heparinum

Lyophilized powder stabilized with approx. 25% (w/w) bovine serum albumin, ≥30 units/mg protein (enzyme + BSA)

Synonyme(s) :

Heparin Lyase III, Heparitinase I, Heparitinase from Flavobacterium heparinum

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Numéro CAS:
37290-86-1
Numéro de classification (Commission des enzymes):
4.2.2.8 (BRENDA, IUBMB)
Numéro MDL:
MFCD00131319
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Source biologique

bacterial (Flavobacterium heparinum)

Niveau de qualité

200

Conjugué

conjugate (Glucosaminoglycan)

Forme

lyophilized powder

Activité spécifique

≥30 units/mg protein (enzyme + BSA)

Poids mol.

70.8 kDa

Application(s)

diagnostic assay manufacturing

Température de stockage

−20°C

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Application

Heparinase III from Flavobacterium heparinum has been used to cleave specific GAGs. It has been used to study its effect on the electrophoretic mobility of the protein reacting with the anti-aFGF (acidic fibroblast growth factor) antibody.

Actions biochimiques/physiologiques

Heparinase III prevents the formation of new blood vessels in vivo. It specifically degrades heparin sulphate at the HNY-I and HNY,6X-G2 links. Unlike other heparinise, heparinase III does not contain the amino acid cysteine. It contains 13 histidine residues, which is responsible for its catalytic action. This enzyme is not active towards heparin or low molecular weight heparins.
Heparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate.

Définition de l'unité

One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units. Package sizes are sold in Sigma units.

Autres remarques

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

FGF binding by extracellular matrix components of Wharton's jelly
Malkowski A, et al.
Acta Biochimica Polonica, 54(2), 357-357 (2007)
Fluid shear stress primes mouse embryonic stem cells for differentiation in a self-renewing environment via heparan sulfate proteoglycans transduction
Toh YC and Voldman J
Faseb Journal, 25(4), 1208-1217 (2011)
Md Nasimuzzaman et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 20(6), 1158-1166 (2012-03-22)
Foamy viruses (FVs) (spumaretroviruses) are good alternative to retroviruses as gene therapy vector. Despite four decades since the discovery of FV, its receptor molecule is still unknown. FV vector transduction of human CD34(+) cells was inhibited by culture with fibronectin.
Sanjai S
Applied Microbiology, 129-129 (2015)
Andrea Garolla et al.
Human reproduction (Oxford, England), 27(4), 967-973 (2012-02-09)
Human papillomavirus (HPV) infection has been demonstrated in the sperm of a large percentage of sexually active males and is associated with an impairment of sperm parameters, with a particular negative impact on sperm motility, suggesting a possible role in
Afficher tous les articles scientifiques apparentés

Articles

Carbohydrate Analysis

A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique