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GE29-0005-93

His Spintrap™ TALON®

Cytiva 29-0005-93

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

ligand

TALON® Superflow Cobalt-IMAC

Fabricant/nom de marque

Cytiva 29-0005-93

Conditionnement

pkg of 50 x 100 μL

Volume du lit

100 μL

Matrice

6% cross-linked agarose

Taille des particules

60-160 μm

cleaning

2-14(Co2+-stripped medium)

working range

3-12(Co2+-stripped medium)

Capacité

≤1 mg binding capacity(histidine-tagged)
 ≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)

Température de stockage

2-8°C

Catégories apparentées

Description générale

His SpinTrap™ Talon® is a single-use column for rapid screening with small samples of histidine-tagged proteins.

Application

His SpinTrap™ Talon® is designed for high purity, small-scale purification and screening of histidine-tagged Proteins from clarified cell lysates. His SpinTrap™ Talon® columns are used together with a standard microcentrifuge and purification takes approximately 10 minutes. The columns make parallel preparations of few or many small-scale cell lysates quick and easy. Prepacked columns enable highly reproducible column to column purification and yield, with RSD < 6%. Leakage of cobalt ions from Talon® Superflow medium is negligible.

Caractéristiques et avantages

  • Suitable for purification of multiple samples in parallel, such as screening.
  • Convenient, ready-to-use single-use spin column, prepacked with 100 μL Talon® Superflow cobalt-IMAC (immobilized metal affinity chromatography) medium offering a different selectivity compared to nickel-charged media.
  • With a microcentrifuge, one run takes about 10 minutes.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

SpinTrap is a trademark of Cytiva
Talon is a registered trademark of Clontech

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.

Protocoles

Purification of Histidine-Tagged Proteins using TALON® Superflow

This page shows how to purify histidine-tagged proteins using TALON Superflow from GE Healthcare.

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