GE28-9852-55
Protein G GraviTrap™
Cytiva 28-9852-55, pack of 10 × 1 mL
Synonyme(s) :
GraviTrap for Protein G, High Performance GraviTrap, Protein G GraviTrap
About This Item
Produits recommandés
Conditionnement
pack of 10 × 1 mL
Fabricant/nom de marque
Cytiva 28-9852-55
Conditions de stockage
(20% Ethanol)
Volume du lit
1 mL
Capacité
~20 mg binding capacity (human IgG)(Protein binding capacity is protein-to-protein dependent.)
Température de stockage
2-8°C
Description générale
Protein G GraviTrap™ are prepacked gravity-flow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on protein G ligands in gravity-flow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.
Application
Caractéristiques et avantages
- Prepacked gravity-flow columns provide convenience, save time, and increase the reliability of your results.
- Purification of antibodies in 30 min allows you to quickly obtain and store your target protein in order to avoid the inherent dangers of protein instability and degradation associated with slow and long purification runs.
- High purity and recovery with purity >95% in one step and recovery typically 70% to 80%.
- High binding capacity such as Protein G GraviTrap™ ~ 20 mg human IgG/column
Stockage et stabilité
Remarque sur l'analyse
Informations légales
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Code de la classe de stockage
3 - Flammable liquids
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Articles
This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from GE Healthcare.
This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.
Protocoles
This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from GE Healthcare
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