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GE28-9505-11

HiScreen™ Q High Performance

Cytiva 28-9505-11

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About This Item

Code UNSPSC :
47101511
Nomenclature NACRES :
NA.56

ligand

quaternary amine

Description

Ion Exchanger Type (value)

Conditionnement

pkg of 1 ea

Fabricant/nom de marque

Cytiva 28-9505-11

Paramètres

44 psi

Taille du lit

7.7 mm × 100 mm

Volume du lit

4.7 mL

D.I. de la colonne

7.7 mm

Matrice

6% cross-linked agarose

Taille des particules

24-44 μm

Taille moy. des particules

34 μm

cleaning

1-14

working range

2-12

Adéquation

suitable for bioprocess medium

Description générale

HiScreen™ Q HP columns are prepacked with Q Sepharose™ High Performance ion exchange chromatography media and are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening.

Application

The small column volume of 4.7 mL and bed height of 10 cm make HiScreen™ columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up. If necessary, two columns can easily be connected in series to give a bed height of 20 cm. Media prepacked in HiScreen™ IEX HP columns are also available in other formats and as bulk packs, making it possible to use the same media for development work, pilot studies, and routine production.

Caractéristiques et avantages

  • Q Sepharose™ High Performance is a strong anion exchange BioProcess medium designed for intermediate and polishing steps in downstream processing
  • Prepacked, ready-to-use columns for convenient process development optimization.
  • 10 cm bed height of HiScreen™ columns is designed to allow method optimization and parameter screening.
  • Easily connected in series to achieve 20 cm bed height.
  • Small bed volume gives fast results and minimal sample/buffer consumption
  • Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

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Articles

HIC as a Capture Step

This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.

Principles and Standard Conditions for Different Purification Techniques

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using GE Healthcare products.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

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Protocoles

HIC Purification Options and Scale up with SOURCE™ Media

This page discusses how to do a purification with SOURCE™ media from GE Healthcare

Sepharose High Performance: Purification with High Resolution

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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