GE28-9042-76
illustra™ Tissue Mini (250)
Cytiva 28-9042-76, sufficient for 250 purifications
About This Item
Produits recommandés
Utilisation
sufficient for 250 purifications
Conditionnement
pkg of 250 ea
Fabricant/nom de marque
Cytiva 28-9042-76
Capacité
>35 μg binding capacity
Catégories apparentées
Description générale
Human and animal genomic DNA is found in the chromosomes and it is much longer and higher in molecular weight than plasmid DNA. Genomic DNA is the code for the genome, and this is what is needed to do genome sequencing.
For sequencing it is desirable that the genomic DNA extraction technique used allows the collection of intact genomic DNA in the longest fragments possible. A kit format can help standardize the procedures used to prepare tissue and cell genomic DNA samples for analysis.
To minimize the incidence of DNA shearing during extraction, Tissue and cells genomicPrep Mini Spin Kit includes a lysis solution with proteinase K to gently release genomic DNA from tissues or cells, including human genomic DNA.
The kit can be used for DNA isolation from diverse tissue types such as liver, kidney, and mouse tails with yields ranging from 0.5 to 1.5 μg of genomic DNA per mg of tissue. The kit consistently generates purified genomic DNA with optical density A260/A280 readings of 1.8 or higher.
The gDNA produced can then be further analyzed in PCR, for SNP genotyping, and next generation sequencing (NGS) projects.
Caractéristiques et avantages
- Rapid procedure produces high molecular weight gDNA with minimal shearing.
- Capable of purifying up to 1.5 µg/mg of tissue from an input tissue sample range of 5 to 5NA mg, and up to 4NA µg of genomic DNA per 5 x 1NA6 cells depending on cell type.
- Total DNA extraction time reduced significantly to 9NA min.
- Produces high-quality genomic DNA with high molecular weight and less degradation.
- Verified in common downstream applications.
Stockage et stabilité
Remarque sur l'analyse
Informations légales
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 3
Certificats d'analyse (COA)
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Articles
Polymerase chain reaction (PCR) is a technique that results in exponential amplification of a target DNA sequence.
Protocoles
This page shows genomic DNA purification from sample applied to FTA cards from GE Healthcare.
This page shows Genomic DNA purification using illustra™ genomicPrep Midi Flow Kits from GE Healthcare.
This page shows Genomic DNA purification using illustra™ genomicPrep Mini Spin Kits from GE Healthcare.
Nucleic Acid Sample Preparation Methodology
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