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GE17-5144-01

HiTrap® Benzamidine Fast Flow

Cytiva 17-5144-01, pack of 1 × 5 mL

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About This Item

Code UNSPSC :
23151816
Nomenclature NACRES :
NA.56

ligand

p-aminobenzamidine

Conditionnement

pack of 1 × 5 mL

Fabricant/nom de marque

Cytiva 17-5144-01

Conditions de stockage

(0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol)

Paramètres

42 psi

Taille du lit

16 mm × 25 mm

Volume du lit

5 mL

D.I. de la colonne

16 mm

Matrice

highly cross-linked 4% agarose

Diamètre moyen

90 μm

cleaning

1-9

Plage de travail

2-8

Température de stockage

2-8°C

Catégories apparentées

Description générale

HiTrap® Benzamidine FF (high sub) are prepacked with Benzamidine Sepharose™ 4 Fast FLow (high sub) and designed specifically for the removal and/or purification of serine proteases such as thrombin.

Application

HiTrap® Benzamidine FF (high sub) are prepacked 1 mL and 5 mL columns for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein. Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.

The medium, Benzamidine Sepharose™ 4 Fast FLow (high sub), is also available as lab packages and is an excellent choice for scale-up. The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC™ System.

Caractéristiques et avantages

  • Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins.
  • Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.
  • HiTrap® Benzamidine FF for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.
  • High binding capacity.
  • The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTA® design or FPLC™ System.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informations légales

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

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Certificats d'analyse (COA)

Lot/Batch Number

Désolés, nous n'avons pas de COA pour ce produit disponible en ligne pour le moment.

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Articles

Principles and standard conditions for different purification techniques

This page provides information about the principles and standard conditions for different purification techniques for the purification of multiprotein complexes with from GE Healthcare.

Troubleshooting of Cleavage Methods

This page shows troubleshooting strategies for cleavage methods using GE Healthcare products.

Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from GE Healthcare.

Cleavage and Purification of GST-Tagged Protein Bound to GSTrap

This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from GE Healthcare.

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Protocoles

Removal of Thrombin and Factor Xa Using HiTrap® Benzamidine FF (High Sub)

This page shows how to remove thrombin and Factor Xa using HiTrap® Benzamidine FF (high sub) from GE Healthcare.

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.

Performing a Separation with GE Healthcare Products Based on GST Fusion Proteins

How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from GE Healthcare.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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