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Key Documents

GE17-5068-01

Sigma-Aldrich

SOURCE™ 15RPC ST 4.6/100

pkg of 1 column

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About This Item

Code UNSPSC :
41115700
Nomenclature NACRES :
SB.52

ligand

matrix surface

Matériaux

polypropylene column

Caractéristiques

autoclavable 20 min at 121°C

Conditionnement

pkg of 1 column

Paramètres

flow rate
0.5 ml/min - 2.5 ml/min flow rate
580 psi

Technique(s)

HPLC: suitable

Taille du lit

4.6 mm × 100 mm

Volume du lit

1.7 mL

D.I. de la colonne

4.6 mm

Matrice

polymeric
polystyrene/divinylbenzene

Groupe de la matrice active

polystyrene-divinylbenzene phase

Taille moy. des particules

15 μm

cleaning

1-14

working range

1-12

Capacité

~10 mg binding capacity

Adéquation

suitable for bioprocess medium

Technique de séparation

reversed phase

Description générale

SOURCE™ 15RPC ST 4.6/100 is a prepacked stainless steel column which provides fast, high-capacity preparative protein purification with reversed phase chromatography.
~10 mg BSA /ml medium at 300 cm/h, ~30 mg bacitracin/ml medium at 300 cm/h, ~50 mg insulin/ml medium at 300 cm/h

Application

SOURCE™ 15RPC is based on rigid, monosized 15 μm diameter polystyrene/divinyl benzene beads. The matrix has outstanding selectivity for RPC. The monodispersity of the beads yields stable beds, Low back pressures and excellent results at high flow rates.With its high physical and chemical stability and very high batch-to-batch reproducibility, SOURCE™ 15RPC is well suited for all stages of an industrial scale operation - from research and process development through scale-up and into production. SOURCE™ 15RPC offers properties superior to those of other polymeric matrices.

Caractéristiques et avantages

  • Wide pH range (1-12), outstanding selectivity, chemical resistance, high capacity, and high resolution at high fLow rates.
  • Excellent scalability, from RESOURCE™ to FineLINE columns.
  • using ÄKTA® design and other high-performance liquid chromatography systems.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol or 70% Acetonitrile)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Produits recommandés

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Informations légales

RESOURCE is a trademark of Cytiva
SOURCE is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Troubleshooting Reversed Phase Chromatography (RPC)

Poor-quality eluent components can cause a phenomenon referred to as “ghosting”. Trace levels of organic impurities bind to the medium, concentrating during equilibration and sample application. When elution begins, these contaminants appear in the chromatogram as unknown, or “ghost” peaks.

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

This page discusses column packing and preparation techniques for reverse phase chromatography

Protocoles

Reverse Phased Chromatography (RPC) in Practice

In this section the practical aspect of Reverse Phased Chromatography ( RPC) is discussed including media and column selection and eluent selection and preparation.

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