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GE17-5055-01

HiTrap® DEAE Fast Flow

Cytiva 17-5055-01, pack of 5 × 1 mL

Synonyme(s) :

DEAE resin, Ion-exchange chromatography resin

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About This Item

Code UNSPSC :
23151816
Nomenclature NACRES :
NA.56

ligand

diethylaminoethyl

Description

Ion Exchanger Type (value)

Conditionnement

pack of 5 × 1 mL

Fabricant/nom de marque

Cytiva 17-5055-01

Paramètres

42 psi

Taille du lit

7 mm × 25 mm

Volume du lit

1 mL

D.I. de la colonne

7 mm

Matrice

6% cross-linked agarose

Taille des particules

45-165 μm

Diamètre moyen

90 μm

cleaning

2-14

working range

2-12

Adéquation

suitable for bioprocess medium

Description générale

HiTrap® DEAE FF is prepacked with DEAE Sepharose Fast Flow, and is a weak anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

Application

Q, SP, DEAE, and CM Sepharose Fast FLow are based on a robust, 6% highly cross-linked beaded Agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast FLow (high sub) is based on 4% highly cross-linked beaded Agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass Proteins.


The active end of the charged group is the same for DEAE Sepharose Fast FLow and ANX Sepharose Fast FLow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast FLow has a diethylaminoethyl-group bound to the Agarose whilst ANX Sepharose 4 Fast FLow (high sub) has a diethylaminopropyl group attached.

Caractéristiques et avantages

  • Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
  • The industry standard for ion exchange chromatography.
  • High fLow rates and good scale-up potential.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
  • Predictable scale-up

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Produit(s) apparenté(s)

Pictogrammes

Flame

Mention d'avertissement

Warning

Mentions de danger

Code de la classe de stockage

3 - Flammable liquids


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

This page shows how to perform a purification of His-tagged membrane proteins.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

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Protocoles

This page covers the use of Sepharose Fast Flow for purification of proteins.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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