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GE17-1408-01

HiTrap® Desalting Columns

Cytiva 17-1408-01, pack of 5 × 5 mL

Synonyme(s) :

Purification Columns

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About This Item

Code UNSPSC :
23151816
Nomenclature NACRES :
NA.56

Conditionnement

pack of 5 × 5 mL

Fabricant/nom de marque

Cytiva 17-1408-01

Conditions de stockage

dry media

Paramètres

42 psi

Taille du lit

16 mm × 25 mm

Volume du lit

5 mL

D.I. de la colonne

16 mm

Matrice

cross-linked dextran

Taille des particules

>20 μm (dry)

cleaning

2-13

Plage de travail

2-13

Adéquation

suitable for bioprocess medium

Catégories apparentées

Description générale

HiTrap® Desalting is a ready-to-use 5 mL column, prepacked with Sephadex® G-25 Superfine, for fast and convenient desalting and buffer exchange.

HiTrap® Desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i.e., buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants or removal of reagents to terminate a reaction.

Application

Desalting and buffer exchange with Sephadex® G-25.

Caractéristiques et avantages

  • Handles sample volumes of 1.5 mL, or larger volumes by connecting up to five columns in series.
  • Replaces dialysis as the method of choice for desalting.
  • Desalting requires less than one minute due to the high rigidity of Sephadex® G-25 and the optimized packing technology.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informations légales

HiTrap is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number

Désolés, nous n'avons pas de COA pour ce produit disponible en ligne pour le moment.

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Articles

General Considerations for Purification of GST-tagged Protein

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

Performing a purification and on-column refolding of an insoluble histidine-tagged protein

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.

Purification of Membrane Proteins

This page shows how to perform a purification of His-tagged membrane proteins.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Afficher tout

Protocoles

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

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