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GE17-0973-10

Phenyl Sepharose™ 6 Fast Flow /High Sub

Cytiva 17-0973-10, pack of 25 mL

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

ligand

phenyl

Conditionnement

pack of 25 mL

Fabricant/nom de marque

Cytiva 17-0973-10

Matrice

6% cross-linked agarose

Taille des particules

45-165 μm

Diamètre moyen

90 μm

cleaning in place

2-14

Plage de travail

3-13

Adéquation

suitable for bioprocess medium

Catégories apparentées

Description générale

Phenyl Sepharose™ 6 Fast FLow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) medium for capture and intermediate purification requiring low to medium hydrophobicity.

Application

Phenyl Sepharose™ 6 Fast FLow is part of the Sepharose™ Fast FLow HIC platform, which has been an industrial standard for HIC chromatography during recent decades.
Phenyl Sepharose™ 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with standard aromatic phenyl groups via uncharged, chemically-stable ether linkages. Two levels of ligand substitution degree help to find the optimal selectivity and binding capacity for a given application.

Phenyl Sepharose™ 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Phenyl Sepharose™ 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Caractéristiques et avantages

  • Standard aromatic HIC media based on Sepharose™ Fast FLow base matrix derivatized via uncharged, chemically-stable ether linkages.
  • Designed for initial and intermediate purification requiring Low to medium hydrophobicity
  • Two levels of substitution help find optimal selectivity and binding capacity for a given application
  • BioProcess medium supported for industrial applications and well-established in approved processes
  • The hydrophilic nature of the base matrix ensures Low levels of non-specific binding.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Sepharose is a trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

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Certificats d'analyse (COA)

Lot/Batch Number

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Articles

Salt Selection and Buffer Preparation

This section is about salt selection and buffer preparation in HIC medium from GE Healthcare, parameters such as buffer ions and pH are considered

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

This page discusses column packing and preparation techniques for reverse phase chromatography

HIC as a Capture Step

This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.

Column, Media and Sample Preparation for Hydrophobic Interaction Chomatography

Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with GE Healthcare media are detailed, including sample application, load volume and temperature

Protocoles

Selectivity and the Properties of a HIC Medium

While ligands contribute significantly to the degree of hydrophobicity of a medium, the matrix can also influence the final selectivity. Chromatography media for hydrophobic interaction are made from porous matrices, chosen for their physical stability, their chemical resistance to stringent cleaning conditions and their low level of non-specific interaction.

HIC Purification Options and Scale up with SOURCE™ Media

This page discusses how to do a purification with SOURCE™ media from GE Healthcare

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