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GE17-0529-01

Calmodulin-Sepharose™ 4B

Cytiva 17-0529-01, pack of 10 mL

Synonyme(s) :

Calmodulin Affinity Resin, Calmodulin-Sepharose

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

ligand

calmodulin

Conditionnement

pack of 10 mL

Fabricant/nom de marque

Cytiva 17-0529-01

Conditions de stockage

(20% Ehtanol)

Matrice

4% agarose

Taille des particules

45-165 μm

Diamètre moyen

90 μm

cleaning in place

4-9

Plage de travail

4-9

Température de stockage

2-8°C

Description générale

Calmodulin Sepharose™ 4B affinity media for purification of proteins with affinity for calmodulin, a highly conserved regulatory protein involved in many cellular processes in eukaryotic cells.

Calmodulin is a highly conserved regulatory protein found in all eukaryotic cells.This protein is involved in many cellular processes such as glycogen metabolism,cytoskeletal control, neurotransmission, phosphate activity and control of NAD?/NADP? Calmodulin binds proteins principally through their interactions with hydrophobic sites on its surface. These sites are exposed after a conformational change induced by the action of Ca2+ on separate Ca2+-binding sites. The binding of enzymes may be enhanced if the enzyme substrate is present and enzyme-substratecalmoldulin-Ca2+ complexes are particularly stable.

Caractéristiques et avantages

  • For single-step purification of native calmodulin-binding proteins
  • Suitable for tandem affinity purification (TAP) of protein complexes
  • Purification of calmodulin-regulated proteins from all eukaryotic cells

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Sepharose is a trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

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Certificats d'analyse (COA)

Lot/Batch Number

Désolés, nous n'avons pas de COA pour ce produit disponible en ligne pour le moment.

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Mahmoud Hajj Chehade et al.
Cell chemical biology, 26(4), 482-492 (2019-01-29)
Ubiquinone (UQ) is a polyprenylated lipid that is conserved from bacteria to humans and is crucial to cellular respiration. How the cell orchestrates the efficient synthesis of UQ, which involves the modification of extremely hydrophobic substrates by multiple sequential enzymes

Articles

Purifcation or Removal of Serine Proteases, e.g. Thrombin and Trypsin, and Zymogens

This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from GE Healthcare.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocoles

Performing a Purification with Calmodulin Sepharose 4B

This page shows how to purify ATPases, adenylate cyclases, protein kinases, phosphodiesterases and neurotransmitters by affinity chromatography using Calmodulin Sepharose 4B from GE Healthcare.

Pull-down assays

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from GE Healthcare.

Column Packing and Preparation for Affinity Chromatography with Specific Groups of Biomolecules

Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.

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Contenu apparenté

Protein Pull-Down

Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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