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Key Documents

GE17-0489-01

Superose® 6 Prep Grade

Cytiva 17-0489-01, pack of 125 mL

Synonyme(s) :

Size exclusion chromatography, Superose 6 column

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About This Item

Code UNSPSC :
41115709
Nomenclature NACRES :
NA.56

Conditionnement

pack of 125 mL

Fabricant/nom de marque

Cytiva 17-0489-01

Matrice

highly cross-linked agarose

Taille des particules

30 μm

cleaning in place

1-14

working range

3-12

Description générale

Good resolution separations of proteins and other biomolecules according to size, Wide separation range – separations in two molecular weight ranges from 1000 up to 5 million, Excellent reproducibility and durability.
Superose® 6 is designed for high-resolution chromatography. Superose® 6 prep grade permits easy, efficient scale-up to preparative separations. Superose® 12 is for high resolution purification of proteins from 1 × 103 to 3 × 105 molecular weight, while Superose® 12 prep grade is useful for preparative purification of these higher molecular weight proteins.

Caractéristiques et avantages

  • Achieve high-resolution separations across exceptionally broad molecular-weight ranges
  • Low ionic strength eluents (< 0.05 M) can utilize a hydrophobic interaction component to alter the selectivity of Superose® for some lipids, peptides, and small aromatic compounds
  • High performance gel filtration media specially designed for preparative purification of biomolecules

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Superose is a registered trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Sigma-Aldrich

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Articles

Setting Column Pressure Limits for Size Exclusion Chromatography

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

Protocoles

Performing a Separation with Superose

Superose from GE Healthcare are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superose column.

Sample Preparation for Size Exclusion Chromatography

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

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