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G75120

Sigma-Aldrich

Sephadex® G-75

Medium

Synonyme(s) :

Gel filtration media, Gel filtration resin

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About This Item

Numéro CAS:
37224-29-6
Numéro MDL:
MFCD00132772
Code UNSPSC :
23151817
Nomenclature NACRES :
NA.56

Niveau de qualité

200

Technique(s)

buffer exchange: suitable

Groupe de la matrice active

phase

Gonflement

1 g swells to 12-15 mL gel

Diamètre des billes

40-120 μm

Application(s)

life science and biopharma

Compatibilité

Cytiva

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Description générale

Sephadex G-75 is widely used for desalting and buffer exchange. This gel filtration resin is created through the crosslinking of dextran with epichlorohydrin. Sephadex varies in its cross-linking level across different types, influencing its swelling capacity and molecular fractionation range.
Sephadex® G-75 is a gel filtration media used in gel filtration chromatography and protein chromatography. Sephadex® G-75 has been used to study cystatin of the mammalian brain, suggest candidates for development of novel oral or other anti-infective agents and to purify procoagulant metalloproteinase from rattlesnake venom.

Application

Sephadex® G-75 has been used to purify low density lipoproteins (LDL) by gel filtration.
Sephadex® G-75 has been used to separate the products from the pepsin digest by gel filtration chromatography and to desalt low density lipoproteins (LDL) by gel filtration.

Caractéristiques et avantages

  • Enables rapid desalting, contaminant removal, and buffer exchange in a single step.
  • Ideal for buffer exchange and cleanup of large biomolecules with a molecular weight exceeding 80,000.
  • Features the highest exclusion limit of 80,000 within the Sephadex G-range.
  • Classic gel filtration resin.

Informations légales

Sephadex is a registered trademark of Cytiva

Remplacé(e)(s) par

Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Inhibition of cyclic AMP-and cyclic GMP-mediated dilations in isolated arteries by oxidized low density lipoproteins.
Galle J, et al.
Arteriosclerosis and Thrombosis, 12(2), 180-186 (1992)
Oxidized low density lipoproteins potentiate vasoconstrictions to various agonists by direct interaction with vascular smooth muscle.
Galle J, et al.
Circulation Research, 66(5), 1287-1293 (1990)
Jose Munoz-Munoz et al.
mBio, 12(4), e0136821-e0136821 (2021-08-04)
The human gut microbiota (HGM) contributes to the physiology and health of its host. The health benefits provided by dietary manipulation of the HGM require knowledge of how glycans, the major nutrients available to this ecosystem, are metabolized. Arabinogalactan proteins
Rapid purification of fluorescent dye-labeled products in a 96-well format for high-throughput automated DNA sequencing.
K Krakowski et al.
Nucleic acids research, 23(23), 4930-4931 (1995-12-11)
Fakhra Amin et al.
Protein and peptide letters, 18(2), 210-218 (2010-11-09)
Cystatins are thiol proteinase inhibitors ubiquitously present in mammalian body and serve various important physiological functions. To purify and characterize Thiol protease inhibitor from buffalo brain and to compare its properties with respect to tissue and organ difference from other
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