G25150

Sephadex^® G-25

Medium

• Synonyme(s) : Sephadex G-25 Medium, Sephadex^® G-25 resins, Size exclusion resins
• Numéro CAS: 9041-35-4
• Numéro MDL: MFCD00132770
• Code UNSPSC : 23151817
• Nomenclature NACRES : NA.56

Propriétés

• Niveau de qualité: 200
• Technique(s): buffer exchange: suitable
• Groupe de la matrice active: phase
• Gonflement: 1 g swells to 4-6 mL
• Taille des billes: 50-150 μm
• Application(s): life science and biopharma
• Compatibilité: Cytiva
• InChI: 1S/C6H12O6.C3H8O3/c7-1-2-3(8)4(9)5(10)6(11)12-2;4-1-3(6)2-5/h2-11H,1H2;3-6H,1-2H2/t2-,3-,4-,5-,6-;/m1./s1
• Clé InChI: BCPVZFFJGCURNR-OCOFDJSDSA-N

Description

Description générale

Sephadex^® G-25 Medium is a size exclusion chromatography or gel filtration resin that is created by crosslinking dextran with epichlorohydrin. The various types of Sephadex can differ in their degree of cross-linking, resulting in variances in their swelling capacity and molecular fractionation range. Sephadex G-25 is one option available among the different G-types of Sephadex. Sephadex G-25 belongs to a range of five G-types, catering to different molecule sizes. Specifically, G-10 is suitable for small molecules, while G-75 is designed to accommodate larger molecules. Sephadex G-25 provides industrial requirements by ensuring a dependable supply and offering extensive technical and regulatory assistance.

Application

Fractionation Range (MW)
Dextrans: 100 - 5,000
Globular Proteins 1,000 - 5,000

Sephadex^® G-25 has been used:

• in the desalting of protease
• in the column for the separation of radioiodinated oTP-1 from free iodine during the characterization of radioiodinated oTP-1 and receptor assay validation

Sephadex^® G-25 has been used to separate the labelled proteins from unreacted dye N-hydroxysuccinimide (NHS) esters by gel permeation chromatography.

Actions biochimiques/physiologiques

Sephadex^® G-25 is a well-established gel filtration resin, widely used for desalting and buffer exchange in industrial applications. Its hydrophilic matrix minimizes nonspecific adsorption, resulting in high recoveries during these processes for proteins and nucleic acids. Sephadex^® G-25 is usually used in affinity chromatography, protein chromatography and gel filtration chromatography.

Caractéristiques et avantages

• Rapid desalting, contaminant removal, and transfer to a new buffer in a single step.
• High recovery rate with minimal sample dilution.
• Offered in prepacked HiPrep Desalting and HiTrap desalting columns for convenient and fast desalting.
• Specifically designed as a BioProcess resin for industrial applications.

Informations légales

Sephadex is a registered trademark of Cytiva

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)