G5160
β-Galactosidase from Aspergillus oryzae
≥8.0 units/mg solid
Synonyme(s) :
β-D-Galactoside galactohydrolase, Lactase from Aspergillus oryzae
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About This Item
Produits recommandés
Forme
solid
Niveau de qualité
Activité spécifique
≥8.0 units/mg solid
Contient
dextrin as extender
Température de stockage
−20°C
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Catégories apparentées
Description générale
β-Galactosidase is a member of the glycoside hydrolase clan A (GH-A) family. It is widely present in microorganisms, which includes both prokaryotes and eukaryotes.
Application
β-Galactosidase from Aspergillus oryzae has been used:
- in in vitro nuclear magnet resonance (NMR)
- as a standard in the β-galactosidase assay to determine the concentration of cell-associated β-galactosidase
- to modify sugar residues on the surface of the mutant slime cells to study the adhesive properties
- for enzymatic hydrolysis of lactose
- for reversed-phase (RP) adsorption
- in the hydrolysis of whey lactose
Actions biochimiques/physiologiques
β-Galactosidase acts on the terminal β-D-galactosyl moieties of disaccharides, glycoconjugates, and polysaccharides. It is useful in mediating transglycosylation reactions such as the synthesis of galactooligosaccharides (GOS). β-Galactosidases might be used for glycobiological and biotechnological applications.
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
Définition de l'unité
1 U hydrolysiert 1.0 μmol o-Nitrophenyl β-D-Galactosid zu o-Nitrophenol und D-Galactose pro Minute beim angegebenen pH- und Temperatur-Wert, falls nicht anders erwähnt.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
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