G4259

β-Glucuronidase from Helix aspersa (garden snail)

Type HA-4

• Numéro de classification (Commission des enzymes): 3.2.1.31 (BRENDA, IUBMB)
• Numéro CE : 232-606-8
• Numéro MDL: MFCD00130629
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Type: Type HA-4
• Forme: partially purified powder
• Activité spécifique: ≥300,000 units/g solid solid
• secondary activity: ≤7,500 units/g solid sulfatase
• Solubilité: H₂O: soluble 1.90-2.10 mg/mL, clear to slightly hazy
• Application(s): clinical testing
• Température de stockage: −20°C

Description

Application

β-glucuronidase was used in enzymic hydrolysis of tissue homogenates for liquid chromatography-electrospray ion trap mass spectrometry (LC/MSⁿ) analysis, to study the structures of degradation products of baicalin.

Actions biochimiques/physiologiques

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

Définition de l'unité

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37°C at the pH 5.0 (30 min assay).

One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

Autres remarques

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H317,H334
• Conseils de prudence: P261 - P280 - P342 + P311
• Classification des risques: Resp. Sens. 1 - Skin Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)