G1270

L-Glutamine Synthetase from Escherichia coli

lyophilized powder, 400-2,000 units/mg protein

• Synonyme(s) : L-Glutamate:ammonia ligase (ADP-forming)
• Numéro CAS: 9023-70-5
• Numéro de classification (Commission des enzymes): 6.3.1.2 (BRENDA, IUBMB)
• Numéro MDL: MFCD00131463
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.26

Propriétés

• Forme: lyophilized powder
• Niveau de qualité: 200
• Activité spécifique: 400-2,000 units/mg protein
• Produit purifié par: affinity chromatography
• Contient: dithioerythritol as preservative
• Composition: Protein, ~5% Lowry
• Solubilité: H₂O: soluble 0.95-1.05 mg/mL, clear to hazy
• Numéro d'accès UniProt: P0A9C5
• Activité étrangère: ATPase <0.2%
• Température de stockage: −20°C
• Informations sur le gène: Escherichia coli K12 ... glnA(948370)

Description

Description générale

L-Glutamine Synthetase from bacteria shows dodecameric structure comprising of 12 active sites. Each active site termed bifunnel, has an ATP and glutamate binding sites. The dodecamer is stabilized by two hexameric rings.

Application

L-Glutamine Synthetase from Escherichia coli has been used in the synthesis of methylglutamine from methylammonium in E coli and in the glutamine synthetase protection activity of human thioredoxin peroxidase enzyme, AOE372.

L-Glutamine synthetase may be used for the purification of proteases from Escherichia coli.

Actions biochimiques/physiologiques

L-glutamine synthetase catalyzes the condensation of L-glutamate and ammonia to L-glutamine. It is a degradative enzyme for glutamic acid.

Nitrogen starvation dictates the expression of the glutamine synthetase (GS) gene in E. coli. GS plays a key role in ammonia assimilation in bacteria. Adenylylation of GS is catalyzed by adenylyltransferase. Adenylylation of GS modulates its catalytic functionality resulting in glutamine limitation in E coli.

Degradative enzyme for glutamic acid

Définition de l'unité

One unit will convert 1.0 μmole of L-glutamate to L-glutamine in 15 min at pH 7.1 at 37 °C.

Forme physique

Contains potassium phosphate, sodium citrate and magnesium acetate buffer salts

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)