G0792

Granulocyte-Macrophage Colony-Stimulating Factor from rat

GM-CSF, recombinant, expressed in E. coli

• Synonyme(s) : GM-CSF
• Numéro MDL: MFCD01868356
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: rat
• Niveau de qualité: 100
• Produit recombinant: expressed in E. coli
• Pureté: >97% (SDS-PAGE)
• Forme: lyophilized powder
• Puissance: 0.03-0.1 ng/mL
• Qualité: endotoxin tested
• Poids mol.: 14.7 kDa
• Conditionnement: pkg of 5X5 μg; pkg of 5 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Impuretés: <1 EU/μg
• Numéro d'accès UniProt: P48750
• Température de stockage: −20°C
• Informations sur le gène: rat ... CSF2(116630) , Csf2(116630)

Description

Actions biochimiques/physiologiques

Four distinct colony-stimulating factors (CSFs) promoting survival, proliferation and differentiation of bone marrow precursor cells have been well characterized: granulocyte-macrophage-CSF (GM-CSF), granulocyte-CSF (G-CSF), macrophage-CSF (M-CSF), and interleukin-3 (IL-3, Multi-CSF). GM-CSF is a multipotential growth factor, stimulating proliferation of progenitor cells from more than one hematopoietic lineage (granulocyte, macrophage, and eosinophil). GM-CSF stimulates colony formation from pluripotent progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. GM-CSF induces myeloid progenitor cells from bone marrow to form colonies containing macrophages and granulocytes in a semisolid media. It is produced by various cell types (activated T cells, B cells, macrophages, mast cells, endothelial cells, and fibroblasts) in response to cytokine and inflammatory stimuli. In addition to granulocyte-macrophage progenitors, GMCSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF can induce nonhematopoietic cells such as endothelial cells to migrate and proliferate. It also stimulates the proliferation of tumor cell lines, including osteogenic sarcoma, carcinoma, and adenocarcinoma cell lines. GM-CSF exerts its biological effects through binding to specific cell surface receptors. The high affinity GM-CSF receptors contain a GM-CSF-specific α?chain and a common α ?chain.

Forme physique

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.25 mg bovine serum albumin.

Notes préparatoires

Recombinant Rat Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is produced from a DNA sequence encoding mature rat GM-CSF. This recombinant protein is a mixture of two rat GM-CSFs, the 127 amino acid form and the 128 amino acid methionyl form. Mature rat GM-CSF has a calculated mass of 14.7 kDa. GM-CSF, an acidic glycoprotein, is species-specific.

Remarque sur l'analyse

The biological activity is measured by its ability to induce proliferation of the factor-dependent mouse cell line, DA3.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable