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Key Documents

FP0010

Sigma-Aldrich

FluoroProfile Protein Quantification Kit

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About This Item

Code UNSPSC :
12352200

Conditions d'expédition

wet ice

Température de stockage

−20°C

Application

Reversible covalent binding to proteins by the fluorophore epicocconone provides a powerful and sensitive method for protein quantification

Caractéristiques et avantages

  • Yields an intense fluorescent red on protein binding
  • Binding is completely reversible making the protein suitable for mass spectrometry and functional assays
  • Water soluble proteins may be quantified without using organic solvents
  • A large dynamic range, large Stokes shift and highly sensitive
  • Highly tolerant of substances commonly interfering with protein quantification
  • Biodegradable and environmentally friendly

Autres remarques

For the quantification of total protein in solution by the interaction of a unique fluorophore with the amines of polypeptides.

Informations légales

FluoroProfile is a trademark of Sigma-Aldrich Co. LLC

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS

  • P5619Protein Standard, analytical standard, 2 mg/vial BSAFDS

Pictogrammes

Exclamation mark

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Eye Irrit. 2

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Phillip J L Bell et al.
Journal of the American Chemical Society, 125(31), 9304-9305 (2003-08-02)
Epicocconone represents a new class of natural fluorescent probes based on a polyketide skeleton isolated from the fungus Epicoccum nigrum. Epicocconone is a small, cell permeable natural product with a high molar absorbtivity and a long Stokes' shift that will
Ondřej Kaplan et al.
PloS one, 11(10), e0163697-e0163697 (2016-10-08)
We describe the production of a highly-active mutant VEGF variant, α2-PI1-8-VEGF121, which contains a substrate sequence for factor XIIIa at the aminoterminus designed for incorporation into a fibrin gel. The α2-PI1-8-VEGF121 gene was synthesized, cloned into a pET-32a(+) vector and
R P Ubrihien et al.
Archives of environmental contamination and toxicology, 82(4), 520-538 (2022-04-21)
The effects of multigenerational Cu exposure on the freshwater gastropod Isidorella newcombi were investigated. Snails were exposed to a range of treatment-specific Cu concentrations in the parental to F2 generations, and a common Cu concentration in the F3 generation. In
Helena A Schmitz et al.
Aquatic toxicology (Amsterdam, Netherlands), 160, 22-30 (2015-01-13)
Metals are accumulated by filter feeding organisms via water, ingestion of suspended sediments or food. The uptake pathway can affect metal toxicity. Saccostrea glomerata were exposed to cadmium through cadmium-spiked suspended sediments (19 and 93μg/g dry mass) and cadmium-enriched phytoplankton
Anne M Taylor et al.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 166, 34-43 (2014-07-11)
The metalloid selenium is an essential element which at slightly elevated concentrations is toxic and mutagenic. In Australia the burning of coal for power generation releases selenium into estuarine environments where it accumulates in sediments. The relationship between selenium exposure

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