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Key Documents

DUO94005

Sigma-Aldrich

Duolink® flowPLA Detection Kit - Violet

Duolink® PLA kit for Flow Cytometry with Violet Detection

Synonyme(s) :

in situ Proximity Ligation Assay, Flowcytometry-PLA, Protein Protein Interaction Kit

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About This Item

Code UNSPSC :
41105331
Nomenclature NACRES :
NA.41

Gamme de produits

Duolink®

Technique(s)

flow cytometry: suitable
immunofluorescence: suitable
proximity ligation assay: suitable

Fluorescence

λex 390 nm; λem 476 nm

Adéquation

suitable for fluorescence

Conditions d'expédition

dry ice

Température de stockage

−20°C

Application

Specificity

Violet Fluorescence Detection Reagents
Use appropriate laser for ?ex 390 nm excitation
Use appropriate filter for ?em 476 nm emission

Application Note

Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Flow validated antibodies are recommended.

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View full Duolink® product list
Based on proximity ligation assay (PLA), the Duolink® PLA technology allows for endogenous detection of protein interactions, post-translational modifications, and protein expression levels at the single molecule level in fixed cells.

Duolink® flowPLA Detection Kits will enable sensitive detection of proteins, protein-protein interactions, and protein modifications within cell populations by flow cytometry. To perform a Duolink® flowPLA experiment, you will need fixed, suspended cells, two primary antibodies that specifically recognize your proteins of interest, a pair of PLA probes (one 100RXN PLUS and one 100 RXN MINUS), wash buffer and a Duolink® flowPLA Detection Kit. The flowPLA Kits are available with 5 different fluorophores: Violet, Green, Orange, Red, or FarRed. The flowPLA Kits contain all the necessary reagents to perform the amplification and detection of bound PLA probes by flow cytometry. Analysis is carried out using standard flow cytometry assay equipment. User must provide a fixed cell suspension, primary antibodies, and corresponding PLA Probes.

Follow the Duolink® PLA Flow Cytometry Protocol to use this product.

Visit our Duolink® PLA Flow Cytometry page on how to run a Duolink® flow experiment, applications, troubleshooting, and more.

Caractéristiques et avantages

  • Analyze protein protein interactions with flow cytometry readout
  • Analyze cell populations with Proximity Ligation Assay
  • Increased sensitivity due to rolling circle amplification for low abundant targets
  • No overexpression or genetic manipulation required
  • Relative quantification possible
  • Works with any flow cytometer instrumentation
  • Easy to follow flexible protocol
  • Publication-ready results

Composants

This product is comprised of the following:
  • 5x Detection Solution - Violet (DUO84005)
  • 5x Ligation Buffer (DUO82009)
  • 5x Amplification Buffer (DUO82050)
  • Ligase (1U/μL)
  • Polymerase (10U/μL)

See datasheet for more information.

Informations légales

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Conseils de prudence

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

10 - Combustible liquids


Certificats d'analyse (COA)

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Articles

Considerations for proper experimental design, preparation and execution of the Duolink® PLA for flow cytometry protocol.

Duolink® Proximity Ligation Assay kits for Flow Cytometry

General tips and tricks for proper experiment execution, aid in identifying potential problems, and provide solutions to ensure a successful Duolink® PLA experiment for flow cytometry.

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