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D7304

Sigma-Aldrich

3,3′-Diaminobenzidine (DAB) Liquid Substrate System tetrahydrochloride

Synonyme(s) :

Chromogenic substrate, DAB substrate solution

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Forme

liquid

Niveau de qualité

200

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme and is useful as a staining agent in immunohistological and immunoblotting techniques. The end product is resistant to alcohol therefore, a variety of counterstains and mounting media can be used with the DAB Liquid Substrate System. The DAB Liquid Substrate System provides all the chromogen and buffer/peroxide solutions needed to produce a fast and convenient DAB substrate solution..

Application

3,3′-Diaminobenzidine (DAB) Liquid Substrate System tetrahydrochloride has been used as a substrate:
  • in the immunoblot assay carried out to identify the changes in heat shock proteins (Hsps) expressed in the liver
  • for visualization of sections after immunostaining
  • in immunohistochemistry of mice knee joint sections
  • in the immunolabeling of kisspeptin neurons in the preoptic area of camel
It produces a brown precipitate with peroxidase for use in immunohistological and immunoblotting staining procedures. Not recommended for ELISA (microwell) procedures.

Attention

Stable at least one year at 2-8 °C.

Quantité

Each DAB Liquid Substrate System will provide 250 mL of DAB substrate solution.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Composants de kit seuls

Réf. du produit
Description
  • 10X DAB Liquid Chromogen 25 mL
  • ready-to-use buffer/peroxide solution 250 mL

Pictogrammes

FlameSkull and crossbonesHealth hazard
GHS02,GHS06,GHS08

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Carc. 1B - Flam. Liq. 2 - STOT SE 1

Organes cibles

Eyes,Central nervous system

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 3

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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S R Pitkin et al.
Behavioural brain research, 119(2), 167-177 (2001-02-13)
The present study aimed to assess the role of advanced age in the development and manifestation of thiamine deficiency using an animal model of Wernicke-Korsakoff syndrome (WKS). Interactions between pyrithiamine-induced thiamine deficiency (PTD) and age were examined relative to working
K M Mader et al.
Genes, brain, and behavior, 7(7), 740-753 (2008-06-04)
Mucopolysaccharidosis type IIIA (MPS IIIA) is an inherited neurodegenerative lysosomal storage disorder characterised by progressive loss of learned skills, sleep disturbance and behavioural problems. Reduced activity of sulphamidase (SGSH; EC 3.10.1.1) results in intracellular accumulation of heparan sulphate (HS), with
Gamze Tanriover et al.
Clinical breast cancer, 18(5), e1067-e1076 (2018-05-29)
Increased S100A8/A9 expression in Gr1-positive cells has been shown in myeloid-derived suppressor cells and may play a role in the formation of a metastatic milieu. We aimed to determine S100A8/A9 expression alone and with coexpression of Gr1 (a myeloid marker)
J Balthazart et al.
Neuroscience, 153(4), 944-962 (2008-05-02)
Songbirds produce learned vocalizations that are controlled by a specialized network of neural structures, the song control system. Several nuclei in this song control system demonstrate a marked degree of adult seasonal plasticity. Nucleus volume varies seasonally based on changes
Jennifer Walshe et al.
Developmental biology, 264(2), 522-536 (2003-12-04)
Characterisation of human craniofacial syndromes and studies in transgenic mice have demonstrated the requirement for Fgf signalling during morphogenesis of membrane bone of the cranium. Here, we report that Fgf activity is also required for development of the oro-pharyngeal skeleton
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Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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