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D4812

Sigma-Aldrich

REDAccuTaq® LA DNA Polymerase

High fidelity Taq with inert dye, 10X buffer included

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About This Item

Numéro MDL:
MFCD01092095
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.55

Niveau de qualité

200

Forme

liquid

Utilisation

sufficient for 250 reactions

Caractéristiques

Long & Accurate PCR
dNTPs included: no
hotstart: no

Concentration

1 unit/μL

Technique(s)

PCR: suitable

Couleur

red

Entrée

purified DNA

Conditions d'expédition

wet ice

Température de stockage

−20°C

Catégories apparentées

Description générale

REDAccuTaq LA DNA polymerase allows for quick recognition in high throughput applications as well as direct loading of amplification products onto agarose gels for electrophoresis. The inert red dye has no effect on automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. However, the PCR product is easily separated from the dye by standard purification methods.
REDAccuTaq® long and accurate (LA) DNA Polymerase is a blend of Taq DNA polymerase and a small amount of thermostable proofreading polymerase that exhibits 3′→5′ exonuclease and an inert red dye that acts as a tracer and loading buffer. It is suitable for amplifying complex DNA templates like human genomic DNA.

Application

REDAccuTaq® LA DNA Polymerase produce long DNA amplicons for:
  • Mutation analysis
  • Cloning genes
  • DNA sequencing
  • cDNA library generation
  • Amplicon sequencing

Caractéristiques et avantages

  • Increased fidelity, up to 6.5× that of Taq DNA polymerase
  • Efficiently and accurately produce amplicons up to 22 kb on genomic templates and up to 40 kb on less complex templates such as lambda or bacterial DNA
  • Visual confirmation that enzyme has been added, and proper mixing has occurred
  • No additional loading dyes needed. A post-reaction aliquot can be directly used on an agarose gel for electrophoresis

Conditionnement

The enzyme is provided with an optimized 10× reaction buffer for enhanced amplification of complex templates.

Définition de l'unité

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

Autres remarques

Store REDAccuTaq LA DNA Polymerase and AccuTaq LA 10x Buffer at –20 °C.

Informations légales

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
REDAccuTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Sarah L Batt et al.
Journal of clinical microbiology, 43(6), 2656-2661 (2005-06-16)
Serotyping Streptococcus pneumoniae is a technique generally confined to reference laboratories, as purchasing pneumococcal antisera is a huge investment. Many attempts have been made to modify serological agglutination techniques to make them more accessible, and more recently developments in serotyping
Claudia Knief et al.
Applied and environmental microbiology, 71(7), 3826-3831 (2005-07-08)
Three upland soils from Thailand, a natural forest, a 16-year-old reforested site, and an agricultural field, were studied with regard to methane uptake and the community composition of methanotrophic bacteria (MB). The methane uptake rates were similar to rates described
Matthew J Ellis et al.
Breast cancer research and treatment, 119(2), 379-390 (2009-10-22)
Mutations in the alpha catalytic subunit of phosphoinositol-3-kinase (PIK3CA) occur in approximately 30% of ER positive breast cancers. We therefore sought to determine the impact of PIK3CA mutation on response to neoadjuvant endocrine therapy. Exons 9 (helical domain) and 20
Maren Godmann et al.
Mechanisms of development, 126(8-9), 650-664 (2009-06-23)
Krüppel-like factor 4 (Klf4, GKLF) was originally characterized as a zinc finger transcription factor essential for terminal differentiation and cell lineage allocation of several cell types in the mouse. Mice lacking Klf4 die postnatally within hours due to impaired skin
S Cheng et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(12), 5695-5699 (1994-06-07)
We have used the polymerase chain reaction (PCR) to amplify up to 22 kb of the beta-globin gene cluster from human genomic DNA and up to 42 kb from phaga lambda DNA. We have also amplified 91 human genomic inserts

Articles

Long and Accurate PCR

Long and accurate PCR applications address the needs for longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase.

Protocoles

Applications with REDTaq™

Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.

End Point PCR Protocol for Long and Accurate DNA Amplification

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.

Long and Accurate PCR Amplification of DNA with RedAccuTaq® (D4812)

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. Red dye allows direct loading of reaction on a gel. REDAccuTaq LA

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