CLL1218
U2OS LMNB1-TUBA1B-ACTB
human female bone (Disease source: Osteosarcoma)
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About This Item
Code UNSPSC :
41106514
Nomenclature NACRES :
NA.81
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product name
U2OS LMNB1-TUBA1B-ACTB,
Source biologique
human female bone (Source Disease: Osteosarcoma)
Niveau de qualité
Température de stockage
−196°C
Informations sur le gène
human ... ACTB(60) , LMNB1(4001) , TUBA1B(10376)
Description générale
U2OS LMNB1-TUBA1B-ACTB are bone osteosarcoma cells from a human 15 year old caucasion female having three distinct ZFN modifications creating a BFP-tagged LMNB1 transgene, a GFP-tagged TUBA1B transgene and a RFP-tagged ACTB transgene expressed from their endogenous gene locus.
This cell line was derived from ATCC Catalog No. HTB-96.
This cell line was derived from ATCC Catalog No. HTB-96.
Application
This product is a human U2OS cell line in which three genomic loci - LMNB1, TUBA1B, and ACTB - have been endogenously tagged with fluorescent protein genes for Blue Fluorescent Protein (BFP), Green Fluorescent Protein (GFP), and Red Fluorescent Protein (RFP), respectively, using CompoZr® zinc finger nuclease (ZFN) technology. Integration resulted in endogenous expression of: 1) the fluorescent fusion protein, BFP-laminB1, easily detected in the nuclear envelope; 2) the fluorescent fusion protein, GFP-α-tubulin, that polymerizes to form characteristic patterns of microtubules; 3) the fluorescent fusion protein, RFP-β-actin, that polymerizes to form characteristic patterns of actin fibers. Imaging of the cell line shows fluorescent labeling of the three proteins having normal cellular distribution patterns which makes the cell line ideal for high content screening to identify compounds that modulate cellular activity. This stable cell line was expanded from a single cell clone. The LMNB1, TUBA1B, and ACTB gene regulation and corresponding protein function are preserved in contrast to cell lines exhibiting overexpression from an exogenous promoter.
To learn more, please visit the Cellular Reporter Cell Line webpage
Actions biochimiques/physiologiques
Nuclear lamin B1, encoded by the LMNB1 gene, is an intermediate filament protein of the nuclear envelope. The protein exhibits transcriptional co-regulatory activity and plays a key role in DNA replication, cellular aging and stress responses. Thus, LMNB1 can be used as a potential biomarker for HCC.
Tubulin α 1b (TUBA1B) is a microtubule protein, which plays a vital role in dynamic process of polymerization and depolymerization during cell replication and division. TUBA1B is overexpressed in hepatocellular carcinoma (HCC) tumor tissues and proliferating HCC cells. This upregulated expression of the gene leads to poor prognosis and chemotherapy resistance in HCC.
Actin β (ACTB) is an abundant cytoskeletal housekeeping protein. The protein is expressed in the nucleus and controls gene expression, cell division and proliferation. ACTB is generally used as a reference gene in measuring expression levels in tumors.
Tubulin α 1b (TUBA1B) is a microtubule protein, which plays a vital role in dynamic process of polymerization and depolymerization during cell replication and division. TUBA1B is overexpressed in hepatocellular carcinoma (HCC) tumor tissues and proliferating HCC cells. This upregulated expression of the gene leads to poor prognosis and chemotherapy resistance in HCC.
Actin β (ACTB) is an abundant cytoskeletal housekeeping protein. The protein is expressed in the nucleus and controls gene expression, cell division and proliferation. ACTB is generally used as a reference gene in measuring expression levels in tumors.
Caractéristiques et avantages
Zinc Finger Nuclease (ZFN)-mediated targeted integration of three different fluorescent tags: LMNB1 is BFP-tagged on chromosome 5q23.2-q31.1, TUBA1B is GFP-tagged on chromosome 12q13.12 and ACTB is RFP-tagged on chromosome 7p22.1 to create a cell line exhibiting stable expression of all three transgenes.
The U2OS cells are adherent, with a doubling time of approx. 29 hours.
The U2OS cells are adherent, with a doubling time of approx. 29 hours.
Qualité
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.
Notes préparatoires
Media Renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3-1:6
The base medium for this cell line is McCoy′s 5A Medium Modified, Cat. No. M9309. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%.
Cell freezing medium-DMSO 1X, Cat. No. C6164.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3-1:6
The base medium for this cell line is McCoy′s 5A Medium Modified, Cat. No. M9309. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%.
Cell freezing medium-DMSO 1X, Cat. No. C6164.
Informations légales
CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d'importation et d'exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L'acheteur (c'est-à-dire l'utilisateur final) est tenu d'obtenir une autorisation d'importation auprès du Ministère français de la Recherche, mentionné à l'article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l'autorisation d'importation requise.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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ACTB in cancer.
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Structural Organization of the Human Gene (LMNB1) Encoding Nuclear Lamin B1
Lin F, et al.
Genomics, 27(2), 230-236 (1995)
Circulating Lamin B1 (LMNB1) Biomarker Detects Early Stages of Liver Cancer in Patients
Sun S, et al.
Journal of Proteome Research, 9(1), 70-78 (2009)
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Lu C, et al.
Digestive Diseases and Sciences, 58(9), 2713-2720 (2013)
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