U-2 OS Cell Line human

92022711, human bone, Not specified

• Synonyme(s) : U-2OS Cells, U2-OS Cells, U20S Cells, U2OS Cells
• Code UNSPSC : 41106514

Propriétés

• Nom du produit: U-2 OS Cell Line human, 92022711, osteosarcoma
• Source biologique: human bone
• Conditionnement: tube of 5 μg 92022711-DNA-5UG; pkg of vial of cells 92022711-1VL
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Not specified
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Osteosarcoma

Description de la lignée cellulaire

Cell line derived in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl.

Application

U-2 OS has been used to study:

• the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA)
• the interaction of human single-stranded DNA binding protein 1 (hSSB1) with bloom syndrome protein helicase (BLM helicase)
• calcium-mediated actin reset (CaAR) in response to physiological changes

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 13
D16S539: 11,12
D5S818: 11
D7S820: 11,12
THO1: 6,9.3
TPOX: 11,12
vWA: 14,18

Milieu de culture

McCoy′s 5a medium with 1.5 mM Glutamine; 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split subconfluent 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new culture flasks.

Autres remarques

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