A375 Cell Line human

88113005, human skin, Epithelial

• Synonyme(s) : A-375 Cells, A375-MEL Cells
• Code UNSPSC : 41106514

Propriétés

• Nom du produit: A375 Cell Line human, 88113005
• Source biologique: human skin
• Description: Human malignant melanoma
• Mode de croissance: Adherent
• Caryotype: Modal no. = 62 (hypotriploid) 9 marker chromosomes. Normal N2, N6 and N22 present.
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human malignant melanoma

Description de la lignée cellulaire

Derived from a 54 year old female with malignant melanoma. The cell line produces rapidly growing amelanotic melanomas in anti-thymocyte serum treated NIH Swiss mice.

Application

A375 cell line has been used to obtain paracrine factors for prolonged culture of mesenchymal stromal cells (MSCs). It has also been used to study oncolytic activity of the peptide LTX-315.

Tumourigenicity studies

Milieu de culture

DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Autres remarques

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