A431 Cell Line human

85090402, human skin, Epithelial

• Synonyme(s) : A-431 Cells, A431/P Cells
• Code UNSPSC : 41106514

Propriétés

• product name: A431 Cell Line human, squamous carcinoma, 85090402
• Source biologique: human skin
• Mode de croissance: Adherent
• Caryotype: Hypertriploid
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Epidermal growth factor (EGF)
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human squamous carcinoma

Description de la lignée cellulaire

Derived from an epidermal carcinoma of the vulva taken from an 85 yr old female. The cells carry large numbers of EGF binding sites and is an indicator cell for anti-TGF binding.

Application

A431 Cell Line human has been used:

• to demonstrate that p38 mitogen-activated protein kinase enhances epidermal growth factor-induced apoptosis in A431 cells
• to study melanoma-specific CD44 (a tumor marker and transmembrane receptor) alternative splicing pattern in A431 cells
• to study the anti-cancer activity of antioxidant-rich Cameroonian medicinal plants

Cell cycle studies

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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