AE17 cell line from mouse lung

10092310, mouse lung, Cells are epithelial-like

• Synonyme(s) : AE17 Cells
• Code UNSPSC : 41106514

Propriétés

• Nom du produit: AE17 cell line from mouse lung, 10092310
• Source biologique: mouse lung
• Description: Mouse malignant mesothelioma
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Cells are epithelial-like
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse malignant mesothelioma

Description de la lignée cellulaire

Mice (C57Black/6J strain female 6-8 weeks old) were exposed to crocidolite asbestos through intraperitoneal injection resulting in tumour development (both ascites and solid tumours). Cultures were established from malignant mesothelial cells obtained from ascites fluid. Cells are tumourigenic in syngeneic immunocompetent mice. Cell Bank Australia reference number: CBA0156

Application

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

Milieu de culture

RPMI1640 (with 2mM L-Glutamine+25mM HEPES) + 5% FCS

Procédure de repiquage

Optimal split ratio 1:16 - 1:32 (seeding density 0.6 x104 cells/cm²).Cells incubated at 37°C with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

Autres remarques

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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Richard Lake - University of Western Australia, Australia