AB12 cell line from mouse lung
10092306, mouse lung, Cells are stellate-shaped
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About This Item
Code UNSPSC :
41106514
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product name
AB12 cell line from mouse lung, 10092306
Source biologique
mouse lung
Description
Malignant mesothelioma from mouse lung
Mode de croissance
Adherent
Caryotype
Not specified
Morphologie
Cells are stellate-shaped
Produits
Not specified
Récepteurs
Not specified
Technique(s)
cell culture | mammalian: suitable
Maladie(s) pertinente(s)
cancer
Conditions d'expédition
dry ice
Température de stockage
−196°C
Origine de la lignée cellulaire
Mouse malignant mesothelioma
Description de la lignée cellulaire
Mice (BALB/c female 6-8 weeks) were exposed to crocidolite asbestos through intraperitoneal injection resulting in tumour development. Cultures were established from malignant mesothelial cells obtained from ascites fluid. Cells are tumourigenic in syngeneic immunocompetent mice. Cell Bank Australia reference number: CBA0146
Application
Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.
Milieu de culture
RPMI 1640 (with 2mM L-Glutamine + 25mM HEPES) + 5% FCS
Procédure de repiquage
Optimal split ratio 1:16 - 1:32 (seeding density 0.8 x104 cells/cm2). Cells incubated at 37°C with 5% CO2.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.
Autres remarques
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Richard Lake - University of Western Australia, Australia
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