MDCK-SIAT1 Cell Line canine

5071502, canine kidney, Epithelial

• Synonyme(s) : MDCK-SIAT1 Cells
• Code UNSPSC : 41106514

Propriétés

• product name: MDCK-SIAT1 Cell Line canine, 05071502
• Source biologique: canine kidney
• Description: Canine Cocker Spaniel Kidney Sialic Acid Over Expression
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Enhanced expression of 6-linked sialic acids
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Canine Cocker Spaniel Kidney Sialic Acid Over Expression

Description de la lignée cellulaire

This cell line has been derived by the stable transfection of MDCK cells with the cDNA of human 2,6-sialtransferase (SIAT1). The cells express two fold?higher amounts of 6-linked sialic acids and two fold-lower amounts of 3-linked sialic acids than parent MDCK cells. MDCK-SIAT1 cells are presented as a suitable system for testing the sensitivity of human influenza virus to neuraminidase inhibitors (NAI) due to their over expression of sialyl-α2,6-galactose moieties.

Milieu de culture

DMEM + 2mM Glutamine + 1mg/ml G418 + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (85-95 %) 1:10 to 1:20 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 37°C; 5% CO₂.

Autres remarques

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