MDCK Cell Line canine

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 84121903, canine kidney, Epithelial

• Synonyme(s) : MDCK (NBL-2) Cells, Madin Darby Canine Kidney Cells, NBL-2 Cells
• Code UNSPSC : 41106514
• eCl@ss : 32190102

Propriétés

• Source biologique: canine kidney
• Forme: cell suspension
• Conditionnement: tube of 5 μg 84121903-DNA-5UG; pkg of vial of cells 84121903-1VL
• Mode de croissance: Adherent
• Caryotype: 2n = 78
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Canine Cocker Spaniel kidney

Description de la lignée cellulaire

From kidney of normal female adult Cocker Spaniel in 1958 by SH Madin and NB Darby (Madin Darby Canine Kidney). Supports growth of wide range of animal viruses: VSV (Indiana strain), infectious Canine Hepatitis, Vaccinia, Coxsackie B5, Adeno and reo viruses, SVEV.

Application

Virus studies: SVEV, VSV, Vaccinia, adeno and reoviruses, Coxsackie B5 et al

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA.

Autres remarques

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