C6079

Collagenase + protease inhibitor

2-5 FALGPA units/mg solid, ≥800 CDU/mg solid

• Synonyme(s) : Collagenase preparation, Protease inhibitor solution
• Numéro de classification (Commission des enzymes): 3.4.24.3 (BRENDA, IUBMB)
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: bacterial (Clostridium histolyticum)
• Niveau de qualité: 200
• Forme: solid
• Activité spécifique: ≥800 CDU/mg solid; 2-5 FALGPA units/mg solid
• Poids mol.: 68-130 kDa
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Informations sur le gène: rat ... LOC296948(296948)

Description

Application

Collagenase with protease inhibitor has been used in a study to assess the association of immune system gene polymorphisms with quantitative features. It has also been used in a study to investigate the quantitative structure-activity relationship study on Clostridium histolyticum collagenase inhibitors. The enzyme from sigma has been used in the isolation of porcine pancreatic islets. It has also been used in the isolation of pancreatic-infiltrating lymphocytes from mice.

Actions biochimiques/physiologiques

Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four grams of calcium (Ca²⁺) per mole of the enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Collagenase treatment can cause some cells to die. Typically, concentrations varying from 0.1 to 5 mg/mL are used for digestion. The duration of reaction can vary from 15 minutes to several hours for satisfactory cell dissociation without causing too much cell death. Zn²⁺ is required for activity. This product is used if the collagenase does not require a significant protease activity.

Autres remarques

Selected lots of collagenase Type XI blended with protease inhibitor to limit the tryptic enzyme activities in pancreatic tissue. The formulation was developed through collaboration with several outside laboratories.

Qualité

Also contains clostripain and non-specific neutral protease activities.

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H302 + H312 + H332,H317,H334
• Conseils de prudence: P261 - P264 - P271 - P280 - P301 + P312 - P302 + P352 + P312
• Classification des risques: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Resp. Sens. 1 - Skin Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Faceshields, Gloves