C2687

Monoclonal Anti-Calponin antibody produced in mouse

clone hCP, ascites fluid

• Synonyme(s) : Anti-Calponin-1
• Numéro MDL: MFCD00164556
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: ascites fluid
• Type de produit anticorps: primary antibodies
• Clone: hCP, monoclonal
• Poids mol.: antigen 34 kDa
• Contient: 15 mM sodium azide
• Espèces réactives: rat, rabbit, pig, human, mouse
• Technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 1:10,000 using human uterus extract
• Isotype: IgG1
• Numéro d'accès UniProt: P51911
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CNN1(1264) , CNN2(1265) ; mouse ... Cnn1(12797) , Cnn2(12798) ; rat ... Cnn1(65204) , Kcnip4(259243)

Description

Spécificité

The antibody hCP (also cited as CALP), localizes calponin in mammalian smooth muscle. In human uterus, an additional band of approximately 27 kDa (l-calponin) may also be stained. The antibody does not cross-react with skeletal, cardiac or non-muscle tissue calponin. Nevertheless, the antibody exhibits cross-reactivity with an epitope (150 kDa range) in human or mouse skeletal muscle. In immunohistochemical staining, the product exhibits smooth muscle specificity. It stains vascular and visceral smooth muscle cells in tissue sections and primary cultured cells (or early passages), but not most cell lines originally derived from smooth muscle. The antibody does not stain epithelial, endothelial or connective tissue fibroblast cells. This product does not cross react with smooth muscle tissue from chicken.

Immunogène

human uterus smooth muscle extract.

Application

Monoclonal Anti-Calponin antibody produced in mouse is suitable for the following applications:

• Immunohistochemistry using formalin-fixed, paraffin-embedded sections
• Immunoprecipitation
• Microarray
• Western blotting (at a dilution of 1:10,000 using human uterus extract)
• Flow cytometry analysis

Actions biochimiques/physiologiques

Calponin is a calcium binding protein that belongs to a family of actin-associated proteins. Calponin is necessary for autophosphorylation of protein kinase C (PKC) in vascular smooth muscle (VSM). Calponin-1 inhibition can prevent uterine smooth muscle cell migration, cause morphological change and rearrange F-actin without affecting its proliferation and apoptosis. Calponin h1 (CN) is a differentiation marker of smooth muscle cells and is down-regulated in the blood vessels of several human tumors. It can inhibit actomyosin ATPase activity. The h1 and h2 calponins bind F-actin and play a key role in regulating actin filaments in smooth muscle and non-muscle cells.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable