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Key Documents

A2054

Sigma-Aldrich

Anti-Monkey IgG (whole molecule)−Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Rabbit Anti-Monkey IgG (whole molecule)-HRP

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

rabbit

Niveau de qualité

Conjugué

peroxidase conjugate

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Espèces réactives

monkey

Technique(s)

direct ELISA: 1:45,000
dot blot: 1:160,000 (direct chemiluminescence assay)

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Catégories apparentées

Description générale

Immunoglobulins (Igs) have two heavy (H) and two light (L) chains, held together by disulphide linkages. They belong to the immunoglobulin super-family. The heavy chain has one variable N-terminal region and three to four constant (CH1-CH4) C-terminal regions. The light chain has one variable and constant region. IgGs are subclassified into IgG1, IgG2, IgG3 and IgG4.
Monkey IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in monkey serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Horseradish Peroxidase (HRP) is an enzyme that catalyzes the conversion of chromogenic substrates such as o-phenylenediamine (OPD), 4-chloro-1-naphthol 3,3′,5,5′-tetramethylbenzidine (TMB), 3,3′-Diaminobenzidine (DAB) or 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS); chemiluminescent substrates such as CPS-3 (enhanced luminal) and fluorogenic substrates such as Ampliflu Red into detectable chromophores, light-emitters or fluorescers, respectively.
Rabbit polyclonal anti-Monkey IgG (whole molecule)−Peroxidase antibody recognizes monkey IgG in vitro and in monkey serum and biological fluids.

Immunogène

monkey IgG (rhesus)

Application

Anti-Monkey IgG (whole molecule)−Peroxidase antibody produced in rabbit has been used in ELISA of serum samples.
Rabbit polyclonal anti-Monkey IgG (whole molecule)−Peroxidase antibody may be used to detect and quantitate the level of IgG in monkey serum and biological fluids via chromogenic, chemoluminescent or fluorogenic immunochemical or immunohistochemical techniques. It may also be used as a secondary antibody in assays that use monkey IgG as the primary antibody. It may be use to detect parasite-specific antibodies in monkey sera, ELISA assays were performed using HRP-conjugated rabbit anti-monkey IgG as the secondary.

Actions biochimiques/physiologiques

Digestion of IgG by papain results in generation of fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallizable (Fc). Fc regions of IgG antibody have enormous therapeutic potential and are exploited for the development of therapeutic antibodies. IgG1 class is the most abundant and its deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions and IgG4 is associated with asymptomatic infection. The IgG levels in monkey show age related increase. In monkeys, IgG subtypes comprises the predominant antibodies during immune response after hepatitis B surface antigen (HBsAg) vaccination. The human and monkey IgG sequences show divergence only in the hinge region and hence exploited for therapeutic antibody testing.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

Notes préparatoires

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Informations légales

Ampliflu is a trademark of Sigma-Aldrich Co. LLC

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

Molecular and functional characterization of cynomolgus monkey IgG subclasses
Jacobsen FW, et al.
Journal of Immunology, 186(1), 341-349 (2011)
Katja Peplinski et al.
Microbiology (Reading, England), 156(Pt 7), 2136-2152 (2010-04-17)
Ralstonia eutropha H16 is probably the best-studied 'Knallgas' bacterium and producer of poly(3-hydroxybutyrate) (PHB). Genome-wide transcriptome analyses were employed to detect genes that are differentially transcribed during PHB biosynthesis. For this purpose, four transcriptomes from different growth phases of the
Analysis of immunoglobulin, complements and CRP levels in serum of captive northern pig-tailed macaques (Macaca leonina)
Zhang XL, et al.
Dong wu Xue Yan Jiu = Zoological Research / "Dong wu Xue Yan Jiu" Bian ji Wei Yuan Hui Bian ji, 35(3), 196-196 (2014)
Renato Porrozzi et al.
The American journal of tropical medicine and hygiene, 71(3), 297-305 (2004-09-24)
This study evaluates cross-immunity in rhesus monkeys (Macaca mulatta) previously infected with one species of Leishmania and have had self-cured disease or were cured by antimony-based therapy upon development of full-blown disease. We found that a self-healing cutaneous leishmaniasis (CL)
Development of humanized monoclonal antibody by logical approach: characterization, functional studies in vitro and immunogenicity studies in vivo in non-human primates
Cheah SH, et al.
Scientific Research and Essays, 7(26), 2288-2299 (2012)

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