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DL-Dithiothréitol solution

1 M in H2O

Synonyme(s) :

(±)-thréo-1,4-Dimercapto-2,3-butanediol solution, DTT

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About This Item

Formule empirique (notation de Hill):
C4H10O2S2
Numéro CAS:
3483-12-3
Poids moléculaire :
154.25
Numéro Beilstein :
1719757
Numéro MDL:
MFCD00004877
Code UNSPSC :
12161504
ID de substance PubChem :
24883472
Nomenclature NACRES :
NA.25

Forme

liquid

Niveau de qualité

100

Pertinence de la réaction

reagent type: reductant

Concentration

1 M in H2O

Densité

1.04 g/mL at 20 °C
1.035 g/mL at 25 °C

Chaîne SMILES 

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

Clé InChI

VHJLVAABSRFDPM-QWWZWVQMSA-N

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Catégories apparentées

Description générale

Dithiothreitol (DTT) is used in proteomics applications to maintain sulfhydryl (-SH) groups in the reduced state and for quantitative reduction of disulfide (-S-S-) groups. It is a frequently used reagent in buffers due to its ability to reduce the oxidation state of a protein sample and preserves enzymatic activity. DTT is oxidized to the cyclic disulfide during the reduction of other disulfides in the solution.
Dithiothreitol is an effective reducing agent for protein analysis. The sealed-ampule format is convenient and easy to use when preparing samples for electrophoresis or other applications and avoids waste and loss of potency.

Application

DL-Dithiothreitol solution has been used:
  • in western blot analysis
  • as a supplement in lysis buffer to study its effects on sperm lysis
  • as a component of cold homogenization buffer, and chilled wash buffer for the isolation of nuclei from frozen rat tissues
  • in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), chromatography, modification of cysteine-containing compounds

Caractéristiques et avantages

Low toxicity and reduced odor compared to other thiol compounds.

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Danger

Mentions de danger

H315 - H318

Classification des risques

Eye Dam. 1 - Skin Irrit. 2

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Sigma-Aldrich

A3221

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Sputolysin® Reagent Intended for the isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum.

Millipore

560000

Sputolysin® Reagent

DTT OmniPur® Grade, >= 99.4%, Used for reduction of protein disulfide linkages

Millipore

3860-OP

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