40839
Anti-Rabbit-IgG - Atto 647N antibody produced in goat
1 mg/mL IgG
Synonyme(s) :
Atto 647N-Anti-Rabbit-IgG antibody produced in goat
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About This Item
Produits recommandés
Conjugué
Atto 647N conjugate
Niveau de qualité
Type de produit anticorps
secondary antibodies
Clone
polyclonal
Forme
liquid
Contient
50% glycerol as stabilizer
Espèces réactives
rabbit
Concentration
1 mg/mL IgG
Technique(s)
immunofluorescence: suitable (5μg/ml)
Fluorescence
λex 647 nm; λem 665 nm in PBS
Adéquation
in accordance for fluorescence
Conditions d'expédition
wet ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Description générale
IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat anti-rabbit IgGs are known to associate with rabbit IgGs.
Immunogène
rabbit IgG
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunofluorescence (1 paper)
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.Atto 647N goat anti-rabbit IgG was used as the secondary antibody for immunofluorescene at a concentration of 5μg/ml on cells fixed in 2% formaldehyde.
Forme physique
Atto 647 goat anti-rabbit IgG (whole molecule) is provided in unit sizes of 1 ml as 1 mg/ml solution in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, containing 5 mM sodium azide as a preservative.
Remarque sur l'analyse
Molar dye/IgG ratio in labeling reaction: ~4:1
free of unconjugated dye
free of unconjugated dye
Informations légales
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Les clients ont également consulté
Till F M Andlauer et al.
eLife, 3 (2014-11-14)
CIDE-N domains mediate interactions between the DNase Dff40/CAD and its inhibitor Dff45/ICAD. In this study, we report that the CIDE-N protein Drep-2 is a novel synaptic protein important for learning and behavioral adaptation. Drep-2 was found at synapses throughout the
Giuliana Fusco et al.
Nature communications, 7, 12563-12563 (2016-09-20)
α-synuclein (αS) is an intrinsically disordered protein whose fibrillar aggregates are the major constituents of Lewy bodies in Parkinson's disease. Although the specific function of αS is still unclear, a general consensus is forming that it has a key role
Anna Albisetti et al.
PLoS pathogens, 13(11), e1006710-e1006710 (2017-11-02)
Trypanosoma brucei belongs to a group of unicellular, flagellated parasites that are responsible for human African trypanosomiasis. An essential aspect of parasite pathogenicity is cytoskeleton remodelling, which occurs during the life cycle of the parasite and is accompanied by major
Masfique Mehedi et al.
Bio-protocol, 7(17) (2017-10-24)
Human respiratory syncytial virus (RSV) infection in human lung epithelial A549 cells induces filopodia, cellular protrusions consisting of F-actin, that extend to neighboring uninfected cells (Mehedi et al., 2016). High-resolution imaging via stimulated emission depletion (STED) microscopy revealed filamentous RSV
Intracellular repair of oxidation-damaged α-synuclein fails to target C-terminal modification sites.
Andres Binolfi et al.
Nature communications, 7, 10251-10251 (2016-01-26)
Cellular oxidative stress serves as a common denominator in many neurodegenerative disorders, including Parkinson's disease. Here we use in-cell NMR spectroscopy to study the fate of the oxidation-damaged Parkinson's disease protein alpha-synuclein (α-Syn) in non-neuronal and neuronal mammalian cells. Specifically
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