18772
Anti-Rabbit IgG - Atto 488 antibody produced in goat
whole molecule, 1 mg/mL protein
Synonyme(s) :
Atto 488-[goat-Anti-rabbit IgG], whole molecule
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46
Produits recommandés
Conjugué
Atto 488 conjugate
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
secondary antibodies
Clone
polyclonal
Contient
50% glycerol
Espèces réactives
rabbit
Concentration
1 mg/mL protein
Technique(s)
immunocytochemistry: 1:500
immunofluorescence: 1:200
immunohistochemistry: suitable
Fluorescence
λex 485 nm; λem 552 nm in PBS
Conditions d'expédition
wet ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Description générale
Goat polyclonal anti-Rabbit IgG (whole molecule)-Atto 488 antibody antibody reats with rabbit IgG in vitro, in rabbit serum and biological fluids.
Rabbit IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rabbit serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Atto 488 is a labeling dye with high molecular absorption (90,000) and quantum yield (0.80) as well as sufficient Stokes shift between excitation and emission maximum. It is optimized for excitation with an argon laser, and is characterized by high photostability. Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Atto 488 is a labeling dye with high molecular absorption (90,000) and quantum yield (0.80) as well as sufficient Stokes shift between excitation and emission maximum. It is optimized for excitation with an argon laser, and is characterized by high photostability. Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Immunogène
Rabbit IgG
Application
Anti-Rabbit IgG has been used for immunocytochemistry applications at a dilution of 1:500 in neural precursor cells.
Goat polyclonal anti-Rabbit IgG (whole molecule)-Atto 488 antibody may be used to detect and quantitate the level of IgG in rabbit serum and biological fluids via fluorogenic immunochemical or immunohistochemical techniques. It may also be used as a secondary antibody in assays that use rabbit IgG as the primary antibody. Atto 488 goat anti-rabbit IgG antibody was used for immunofluorescence to detect L. monocytogenes in mouse tissue sections following incubation with an anti-listeria antibody. Antibody was used at a 1:200 dilution.
Forme physique
Atto 488 goat anti-rabbit IgG (whole molecule) is provided in unit sizes of 1 ml as 1 mg/ml solutions in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, containing 5 mM sodium azide as a preservative.
Remarque sur l'analyse
Unconjugated dye ≤5% of total fluorescence; dye-to-protein ratio >2.
Informations légales
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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